Dynamic control of illumination beam phase profile in a scanning optical microscope

Abhijit Das; Bosanta R. Boruah

doi:10.1117/12.2032672

17 June 2013 Dynamic control of illumination beam phase profile in a scanning optical microscope

Abhijit Das, Bosanta R. Boruah

Proceedings Volume 8797, Advanced Microscopy Techniques III; 87970K (2013) https://doi.org/10.1117/12.2032672
Event: European Conferences on Biomedical Optics, 2013, Munich, Germany

Abstract

We propose a scanning optical microscope, for samples introducing spatially varying aberrations to the illumination beam. It is implemented with a microscope that has binary hologram based beam scanning mechanism where illumination beam phase profile is varied from pixel to pixel. Unlike a conventional scanning microscope, the scanning is achieved by the beam diffracted from a binary hologram written on the display panel of a liquid crystal spatial light modulator. The aberration correction is achieved without a separate wavefront sensor. For correcting the aberration in the illumination beam the signal is maximized by changing the shape of the binary hologram in terms of chosen Zernike mode coefficients.

Citation Download Citation

Abhijit Das and Bosanta R. Boruah "Dynamic control of illumination beam phase profile in a scanning optical microscope", Proc. SPIE 8797, Advanced Microscopy Techniques III, 87970K (17 June 2013); https://doi.org/10.1117/12.2032672

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