Phenothiazinium dyes in association with diode red laser against B16F10 melanoma cells: in vitro study

Anderson F. Miranda; Gustavo M. P. Santos; Susana C. P. S. de Oliveira; Juliana S. C. Monteiro; Fernando J. P. Sampaio; Rafael Araújo Gomes Júnior; Aldo Brugnera Jr.; Maria F. M. Gesteira; Fátima A. A. Zanin; Antônio Luiz Barbosa Pinheiro; Marcos A. Vannier-Santos

doi:10.1117/12.2038466

18 February 2014 Phenothiazinium dyes in association with diode red laser against B16F10 melanoma cells: in vitro study

Anderson F. Miranda, Gustavo M. P. Santos, Susana C. P. S. de Oliveira, Juliana S. C. Monteiro, Fernando J. P. Sampaio, Rafael Araújo Gomes Júnior, Aldo Brugnera Jr., Maria F. M. Gesteira, Fátima A. A. Zanin, Antônio Luiz Barbosa Pinheiro, Marcos A. Vannier-Santos

Author Affiliations +

Proceedings Volume 8932, Mechanisms for Low-Light Therapy IX; 893212 (2014) https://doi.org/10.1117/12.2038466
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

In Brazil solar incidence is high and continuous throughout the year. Body exposure to sunlight may be a key point in the rates of individuals affected by melanoma and other types of skin cancer in many countries. Brazil already occupies the 15th place in the ranking of melanoma cases and the limitations presented by drugs used in the therapy of this cancer, new approaches are being used in an attempt to decrease the mortality of this malignancy. The aim of this study was to evaluate the effects of phenothiazinium dyes (PD) associated with laser light on murine melanoma (B16F10) in vitro by measuring cell growth using colorimetric assay before and after photodynamic therapy. We used a diode laser (λ660nm, 2.4 J/cm², 40 mW, 60 s, CW) associated with PD at 12.5 μg/mL, time pre-irradiation of 30 minutes). The following groups were tested: control (LF-), PD (L-F+), Laser (L+F-), Laser + PD (L+F+). The results showed a significant reduction in cell growth in the group treated by the photodynamic therapy compared to the control at 24 and 48 h (p < 0.001). Were showing at 30 min PD has a dose-dependent response on B16F10 cells, but at 24 h did not demonstrated this response.

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Anderson F. Miranda, Gustavo M. P. Santos, Susana C. P. S. de Oliveira, Juliana S. C. Monteiro, Fernando J. P. Sampaio, Rafael Araújo Gomes Júnior, Aldo Brugnera Jr., Maria F. M. Gesteira, Fátima A. A. Zanin, Antônio Luiz Barbosa Pinheiro, and Marcos A. Vannier-Santos "Phenothiazinium dyes in association with diode red laser against B16F10 melanoma cells: in vitro study", Proc. SPIE 8932, Mechanisms for Low-Light Therapy IX, 893212 (18 February 2014); https://doi.org/10.1117/12.2038466

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