Evaluation of enamel by scanning electron microscopy green LED associated to hydrogen peroxide 35% for dental bleaching

Juliana S. C. Monteiro; Susana C. P. S. de Oliveira; Fátima A. A. Zanin; Gustavo M. P. Santos; Fernando J. P. Sampaio D.D.S.; Rafael Araújo Gomes Júnior; Maria F. M. Gesteira D.D.S.; Marcos A. Vannier-Santos; Antônio Luiz Barbosa Pinheiro

doi:10.1117/12.2038558

18 February 2014 Evaluation of enamel by scanning electron microscopy green LED associated to hydrogen peroxide 35% for dental bleaching

Juliana S. C. Monteiro, Susana C. P. S. de Oliveira, Fátima A. A. Zanin, Gustavo M. P. Santos, Fernando J. P. Sampaio D.D.S., Rafael Araújo Gomes Júnior, Maria F. M. Gesteira D.D.S., Marcos A. Vannier-Santos, Antônio Luiz Barbosa Pinheiro

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Proceedings Volume 8932, Mechanisms for Low-Light Therapy IX; 893214 (2014) https://doi.org/10.1117/12.2038558
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Dental bleaching is a frequently requested procedure in clinical dental practice. The literature is contradictory regarding the effects of bleaching agents on both morphology and demineralization of enamel after bleaching. The aim of this study was to analyze by SEM the effect of 35% neutral hydrogen peroxide cured by green LED. Buccal surfaces of 15 pre-molars were sectioned and marked with a central groove to allow experimental and control groups on the same specimen. For SEM, 75 electron micrographs were evaluated by tree observers at 43X, 220X and 1000X. Quantitative analysis for the determination of the surface elemental composition of the samples through X-ray microanalysis by SEM was also performed. The protocol tested neither showed significant changes in mineral composition of the samples nor to dental enamel structure when compared to controls. SEM analysis allowed inferring that there were marked morphological differences between the enamel samples highlighting the need for the use of the same tooth in comparative morphological studies. The tested protocol did not cause morphological damage the enamel surface when compared to their respective controls.

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Juliana S. C. Monteiro, Susana C. P. S. de Oliveira, Fátima A. A. Zanin, Gustavo M. P. Santos, Fernando J. P. Sampaio D.D.S., Rafael Araújo Gomes Júnior, Maria F. M. Gesteira D.D.S., Marcos A. Vannier-Santos, and Antônio Luiz Barbosa Pinheiro "Evaluation of enamel by scanning electron microscopy green LED associated to hydrogen peroxide 35% for dental bleaching", Proc. SPIE 8932, Mechanisms for Low-Light Therapy IX, 893214 (18 February 2014); https://doi.org/10.1117/12.2038558

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KEYWORDS

Teeth

Scanning electron microscopy

Hydrogen

Dental bleaching

Light emitting diodes

Green light emitting diodes

Statistical analysis

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