Raman study of analysis for the states of maturation of neural cell

Kosuke Hashimoto; Suguru N. Kudoh; Hidetoshi Sato

doi:10.1117/12.2039073

4 March 2014 Raman study of analysis for the states of maturation of neural cell

Kosuke Hashimoto, Suguru N. Kudoh, Hidetoshi Sato

Proceedings Volume 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry; 893914 (2014) https://doi.org/10.1117/12.2039073
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The combination of confocal micro-Raman spectroscopy and multivariate analysis is carried out for analysis of maturation of neurons. This study suggests that Raman data reflects the stages of neural maturation which relates with the expression of new neural function such as spontaneous activity. Neurons obtained from a hippocampus of rat embryos are cultured in a dish with quartz bottom. According to the previous electrophysiological study, matured neural cell network showed regulated pulsation with interval of several seconds without any stimulation. It suggested that alterations in the molecular composition took place in the cell. The Raman measurements are carried out to observe this alteration along with the maturing process of neurons. The spectra of live neural cells measured after 2, 8, 15, 30, 45, 60, 75, 90, 105 and 120 days of culturing are analyzed by principal component analysis (PCA). The result shows several groups suggesting the maturation scheme which is observed by the electrophysiological studies. It demonstrates that the maturation process of neural cells can be monitored by Raman spectroscopy.

Citation Download Citation

Kosuke Hashimoto, Suguru N. Kudoh, and Hidetoshi Sato "Raman study of analysis for the states of maturation of neural cell", Proc. SPIE 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, 893914 (4 March 2014); https://doi.org/10.1117/12.2039073

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available