Based on surface-enhanced Raman spectroscopy analysis of serum albumin in different stages of liver disease for early screening primary liver cancer

Fadian Liao; Qiuyong Ruan; Juqiang Lin; Jinyong Lin; Yongyi Zeng; Ling Li; Zufang Huang; Nenrong Liu; Rong Chen

doi:10.1117/12.2069157

17 September 2014 Based on surface-enhanced Raman spectroscopy analysis of serum albumin in different stages of liver disease for early screening primary liver cancer

Fadian Liao, Qiuyong Ruan, Juqiang Lin, Jinyong Lin, Yongyi Zeng, Ling Li, Zufang Huang, Nenrong Liu, Rong Chen

Author Affiliations +

Proceedings Volume 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014); 923010 (2014) https://doi.org/10.1117/12.2069157
Event: Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 2014, Wuhan, China

Abstract

Despite the introduction of high-technology methods of detection and diagnosis, screening of primary liver cancer (PLC) remains imperfect. To diagnosis PLC earlier, Surface-enhanced Raman spectroscopy (SERS) coupled with cellulose-acetate membrane electrophoresis were introduced to separate human serum albumin and SERS spectra. Three groups (15 normal persons’ samples, 17 hepatitis/cirrhosis samples, 15 cases of PLC) of serum albumin were tested. Silver colloid was used to obtain SERS spectra of human serum albumin. Principal component analysis (PCA) and linear discriminant analysis (LDA) were also employed for statistical analysis. The mean Raman spectra of three groups and the difference spectra of any two suggested that the albumin has changed in liver patients. Compared to normal groups, some Raman peaks have shifted or even disappeared in hepatitis/cirrhosis and PLCs groups. The sensitivity and specificity between PLCs and normal groups is 80% and 93.3%. Among hepatitis/cirrhosis and normal groups, the sensitivity is 88.2% and specificity is also 93.3%. Besides, the sensitivity and specificity between PLCs and hepatitis/cirrhosis groups is 86.7% and 76.5%. All the above data and results indicated that early screening of PLC is potential by SERS in different stages of liver disease before cancer occurs.

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Fadian Liao, Qiuyong Ruan, Juqiang Lin, Jinyong Lin, Yongyi Zeng, Ling Li, Zufang Huang, Nenrong Liu, and Rong Chen "Based on surface-enhanced Raman spectroscopy analysis of serum albumin in different stages of liver disease for early screening primary liver cancer", Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 923010 (17 September 2014); https://doi.org/10.1117/12.2069157

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KEYWORDS

Photonic integrated circuits

Surface enhanced Raman spectroscopy

Raman spectroscopy

Liver

Liver cancer

Silver

Diagnostics

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