Serum albumin analysis for type II diabetes detection using surface-enhanced Raman spectroscopy

Jinyong Lin; Gang Cao; Juqiang Lin; Nenrong Liu; Fadian Liao; Qiuyong Ruan; Shanshan Wu; Zufang Huang; Ling Li; Rong Chen

doi:10.1117/12.2068851

17 September 2014 Serum albumin analysis for type II diabetes detection using surface-enhanced Raman spectroscopy

Jinyong Lin, Gang Cao, Juqiang Lin, Nenrong Liu, Fadian Liao, Qiuyong Ruan, Shanshan Wu, Zufang Huang, Ling Li, Rong Chen

Author Affiliations +

Proceedings Volume 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014); 92301Z (2014) https://doi.org/10.1117/12.2068851
Event: Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 2014, Wuhan, China

Abstract

Surface-enhanced Raman scattering (SERS) spectroscopy combined with membrane electrophoresis (ME) was firstly employed to detect albumin variation in type II diabetic development. Albumin was first purified from human serum by ME and then mixed with silver nanoparticles to perform SERS spectral analysis. SERS spectra were obtained from blood albumin samples of 20 diabetic patients and 19 healthy volunteers. Subtle but discernible changes in the acquired mean spectra of the two groups were observed. Tentative assignment of albumin SERS bands indicated specific structural changes of albumin molecule with diabetic development. Meanwhile, PCA-LDA diagnostic algorithms were employed to classify the two kinds of albumin SERS spectra, yielding the diagnostic sensitivity of 90% and specificity of 94.7%. The results from this exploratory study demonstrated that the EM-SERS method in combination with multivariate statistical analysis has great potential for the label-free detection of albumin variation for improving type II diabetes screening.

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Jinyong Lin, Gang Cao, Juqiang Lin, Nenrong Liu, Fadian Liao, Qiuyong Ruan, Shanshan Wu, Zufang Huang, Ling Li, and Rong Chen "Serum albumin analysis for type II diabetes detection using surface-enhanced Raman spectroscopy", Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 92301Z (17 September 2014); https://doi.org/10.1117/12.2068851

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