Multispectral scanning laser ophthalmoscopy combined with optical coherence tomography for simultaneous in vivo mouse retinal imaging

Pengfei Zhang; Azhar Zam; Yifan Jian; Xinlei Wang; Marie E. Burns; Marinko V. Sarunic; Edward N. Pugh Jr.; Robert J. Zawadzki

doi:10.1117/12.2080468

11 March 2015 Multispectral scanning laser ophthalmoscopy combined with optical coherence tomography for simultaneous in vivo mouse retinal imaging

Pengfei Zhang, Azhar Zam, Yifan Jian, Xinlei Wang, Marie E. Burns, Marinko V. Sarunic, Edward N. Pugh Jr., Robert J. Zawadzki

Author Affiliations +

Proceedings Volume 9307, Ophthalmic Technologies XXV; 93070H (2015) https://doi.org/10.1117/12.2080468
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

A compact, non-invasive multi-modal system has been developed for in vivo mouse retina imaging. It is configured for simultaneously detecting green and red fluorescent protein signals with scanning laser ophthalmoscopy (SLO) back-scattered light from the SLO illumination beam, and depth information about different retinal layers by means of Optical Coherence Tomography (OCT). Simultaneous assessment of retinal characteristics with different modalities can provide a wealth of information about the structural and functional changes in the retinal neural tissue and chorio-retinal vasculature in vivo. Additionally, simultaneous acquisition of multiple channels facilitates analysis of the data of different modalities by automatic temporal and structural co-registration. As an example of the instrument’s performance we imaged the retina of a mouse with constitutive expression of GFP in microglia cells (Cx3cr1^GFP/+), and which also expressed the red fluorescent protein mCherry in Müller glial cells by means of adeno-associated virus delivery (AAV2) of an mCherry cDNA driven by the GFAP (glial fibrillary acid protein) promoter.

Citation Download Citation

Pengfei Zhang, Azhar Zam, Yifan Jian, Xinlei Wang, Marie E. Burns, Marinko V. Sarunic, Edward N. Pugh Jr., and Robert J. Zawadzki "Multispectral scanning laser ophthalmoscopy combined with optical coherence tomography for simultaneous in vivo mouse retinal imaging", Proc. SPIE 9307, Ophthalmic Technologies XXV, 93070H (11 March 2015); https://doi.org/10.1117/12.2080468

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available