Study of acetowhitening mechanisms in live mammalian cells with label-free subcellular-level multimodal nonlinear optical microscopy

Jian Lin; Sengkhoon Teh; Wei Zheng; Zi Wang; Zhiwei Huang

doi:10.1117/12.2080818

5 March 2015 Study of acetowhitening mechanisms in live mammalian cells with label-free subcellular-level multimodal nonlinear optical microscopy

Jian Lin, Sengkhoon Teh, Wei Zheng, Zi Wang, Zhiwei Huang

Author Affiliations +

Proceedings Volume 9329, Multiphoton Microscopy in the Biomedical Sciences XV; 93290T (2015) https://doi.org/10.1117/12.2080818
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

The tissue acetowhitening effect in acetic acid instillation procedure is a simple and economic method for neoplasia detection and has been clinically utilized since 1925. It is suspected that the optical property (e.g. scattering) change in acetowhitening is due to coagulation of intracellular proteins, but no experimental proof has been reported yet. In this work, we use third-harmonic generation (THG) and two-photon excited fluorescence (TPEF) to investigate the acetowhitening phenomenon induced by acidic acid in live mammalian cells without labeling. We studied the acetowhitening effect with different acetic acid concentrations and the co-localized TPEF and THG imaging on tryptophan and NADH at subcellular-level reveals that the acetowhitening phenomenon is highly related with proteins involved in metabolic pathways in the nucleus and cytoplasm in live cells.

Citation Download Citation

Jian Lin, Sengkhoon Teh, Wei Zheng, Zi Wang, and Zhiwei Huang "Study of acetowhitening mechanisms in live mammalian cells with label-free subcellular-level multimodal nonlinear optical microscopy", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93290T (5 March 2015); https://doi.org/10.1117/12.2080818

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