Parallel and flexible imaging using two-photon RESOLFT microscopy with spatial light modulator control

Yi Xue; Christopher J. Rowlands; Peter T. C. So

doi:10.1117/12.2078509

5 March 2015 Parallel and flexible imaging using two-photon RESOLFT microscopy with spatial light modulator control

Yi Xue, Christopher J. Rowlands, Peter T. C. So

Proceedings Volume 9329, Multiphoton Microscopy in the Biomedical Sciences XV; 932927 (2015) https://doi.org/10.1117/12.2078509
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

High resolution imaging in three dimension is important for biological research. RESOLFT (Reversible Saturable Optical Fluorescence Transitions) microscopy is one technique can achieve lateral super-resolution imaging. Twophoton microscopy naturally generate high resolution in the longitudinal direction with less background compared to single photon excitation. In this paper, we combine these two methods to realize three-dimensional high-resolution imaging. Spatial light modulator (SLM) is used as a flexible phase mask of the microscopy. Multiple super-resolution focuses as an array or in arbitrary positions could be generated by phase retrieval. This microscopy by SLM control could applied to parallel two-photon RESOLFT imaging or multiple spots tracking in high-resolution.

Citation Download Citation

Yi Xue, Christopher J. Rowlands, and Peter T. C. So "Parallel and flexible imaging using two-photon RESOLFT microscopy with spatial light modulator control", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 932927 (5 March 2015); https://doi.org/10.1117/12.2078509

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available