Time-resolved study of microorganisms  by Raman spectroscopy

Ota Samek; Andrea Haronikova; Stanislav Obruca; Silvie Bernatova; Jan Jezek; Martin Siler; Katarina Mlynarikova; Pavel Zemanek

doi:10.1117/12.2183950

15 July 2015 Time-resolved study of microorganisms by Raman spectroscopy

Ota Samek, Andrea Haronikova, Stanislav Obruca, Silvie Bernatova, Jan Jezek, Martin Siler, Katarina Mlynarikova, Pavel Zemanek

Author Affiliations +

Proceedings Volume 9537, Clinical and Biomedical Spectroscopy and Imaging IV; 953703 (2015) https://doi.org/10.1117/12.2183950
Event: European Conferences on Biomedical Optics, 2015, Munich, Germany

Abstract

The main goal of our investigations is to focus on the basic physiological mechanisms of microorganisms (yeast and bacteria), exposed to different conditions, by time-resolved Raman spectroscopy. This study provides an insight into the mechanism of targeted stress factors or the influence of different cultivation times on species metabolism in vivo, in realtime and label free. We also focused on time-course study of physico-chemical properties of bacterial cells and cell cytoplasm with respect to the intracellular content of polyhydroxyalkanoates and to the production of yeast lipids or carotenoids.

Citation Download Citation

Ota Samek, Andrea Haronikova, Stanislav Obruca, Silvie Bernatova, Jan Jezek, Martin Siler, Katarina Mlynarikova, and Pavel Zemanek "Time-resolved study of microorganisms by Raman spectroscopy", Proc. SPIE 9537, Clinical and Biomedical Spectroscopy and Imaging IV, 953703 (15 July 2015); https://doi.org/10.1117/12.2183950

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