Automatic segmentation of Leishmania parasite in microscopic images using a modified CV level set method

Maria Farahi; Hossein Rabbani; Ardeshir Talebi; Omid Sarrafzadeh; Shahab Ensafi

doi:10.1117/12.2228580

9 December 2015 Automatic segmentation of Leishmania parasite in microscopic images using a modified CV level set method

Maria Farahi, Hossein Rabbani, Ardeshir Talebi, Omid Sarrafzadeh, Shahab Ensafi

Author Affiliations +

Proceedings Volume 9817, Seventh International Conference on Graphic and Image Processing (ICGIP 2015); 98170K (2015) https://doi.org/10.1117/12.2228580
Event: Seventh International Conference on Graphic and Image Processing, 2015, Singapore, Singapore

Abstract

Visceral Leishmaniasis is a parasitic disease that affects liver, spleen and bone marrow. According to World Health Organization report, definitive diagnosis is possible just by direct observation of the Leishman body in the microscopic image taken from bone marrow samples. We utilize morphological and CV level set method to segment Leishman bodies in digital color microscopic images captured from bone marrow samples. Linear contrast stretching method is used for image enhancement and morphological method is applied to determine the parasite regions and wipe up unwanted objects. Modified global and local CV level set methods are proposed for segmentation and a shape based stopping factor is used to hasten the algorithm. Manual segmentation is considered as ground truth to evaluate the proposed method. This method is tested on 28 samples and achieved 10.90% mean of segmentation error for global model and 9.76% for local model.

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Maria Farahi, Hossein Rabbani, Ardeshir Talebi, Omid Sarrafzadeh, and Shahab Ensafi "Automatic segmentation of Leishmania parasite in microscopic images using a modified CV level set method", Proc. SPIE 9817, Seventh International Conference on Graphic and Image Processing (ICGIP 2015), 98170K (9 December 2015); https://doi.org/10.1117/12.2228580

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