Correlative atomic force and confocal fluorescence microscopy: single molecule imaging and force induced spectral shifts
(Conference Presentation)

Thomas Basché; Gerald Hinze; Sven Stöttinger

doi:10.1117/12.2235213

3 November 2016 Correlative atomic force and confocal fluorescence microscopy: single molecule imaging and force induced spectral shifts (Conference Presentation)

Thomas Basché, Gerald Hinze, Sven Stöttinger

Author Affiliations +

Proceedings Volume 9925, Nanoimaging and Nanospectroscopy IV; 99250M (2016) https://doi.org/10.1117/12.2235213
Event: SPIE Nanoscience + Engineering, 2016, San Diego, California, United States

Abstract

A grand challenge in nanoscience is to correlate structure or morphology of individual nano-sized objects with their photo-physical properties. An early example have been measurements of the emission spectra and polarization of single semiconductor quantum dots as well as their crystallographic structure by a combination of confocal fluorescence microscopy and transmission electron microscopy.[1] Recently, the simultaneous use of confocal fluorescence and atomic force microscopy (AFM) has allowed for correlating the morphology/conformation of individual nanoparticle oligomers or molecules with their photo-physics.[2, 3] In particular, we have employed the tip of an AFM cantilever to apply compressive stress to single molecules adsorbed on a surface and follow the effect of the impact on the electronic states of the molecule by fluorescence spectroscopy.[3] Quantum mechanical calculations corroborate that the spectral changes induced by the localized force can be associated to transitions among the different possible conformers of the adsorbed molecule.

Conference Presentation

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Thomas Basché, Gerald Hinze, and Sven Stöttinger "Correlative atomic force and confocal fluorescence microscopy: single molecule imaging and force induced spectral shifts (Conference Presentation)", Proc. SPIE 9925, Nanoimaging and Nanospectroscopy IV, 99250M (3 November 2016); https://doi.org/10.1117/12.2235213

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KEYWORDS

Confocal microscopy

Molecules

Luminescence

Molecular spectroscopy

Atomic force microscopy

Microscopy

Quantum dots

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