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15 March 2023 Small SPAD-arrays for confocal fluoresence lifetime imaging (Conference Presentation)
Max Tillmann, Felix Koberling, Tino Roehlicke, Michael Wahl, Cyril Saudan, Harald A.R. Homulle, Ivan Michel Antolovic, Rainer Erdmann
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Proceedings Volume PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; PC123840G (2023) https://doi.org/10.1117/12.2655332
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Confocal microscopy is an essential tool in many academic disciplines due to its intrinsic sectioning capability. The combination with time-resolved single photon detectors and Time-Correlated Single Photon Counting (TCSPC) devices has established it as the leading platform for time resolved investigation methods such as fluorescence lifetime imaging (FLIM). Recently, high-performance SPAD-arrays featuring few tens of pixels have become available. In this work we present the two central hardware building blocks: PicoQuant’s latest multi-channel TCSPC device and a cooled high-performance 23-pixel SPAD-array developed jointly with Pi Imaging Technologies. We discuss how these open up new possibilities in time-resolved confocal microscopy.
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Max Tillmann, Felix Koberling, Tino Roehlicke, Michael Wahl, Cyril Saudan, Harald A.R. Homulle, Ivan Michel Antolovic, and Rainer Erdmann "Small SPAD-arrays for confocal fluoresence lifetime imaging (Conference Presentation)", Proc. SPIE PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, PC123840G (15 March 2023); https://doi.org/10.1117/12.2655332
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KEYWORDS
Confocal microscopy
Fluorescence correlation spectroscopy
Fluorescence lifetime imaging
Microscopy
Data processing
Imaging technologies
Signal to noise ratio
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