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We study far from equilibrium systems through investigating how groups of bacteria behave when other bacteria are present in the system and when barriers are introduced. For these studies we use structured light to fabricate microscopic structures for optical trapping and cell studies using two photon photopolymerization process. Structured light is created using spatial light modulator and correct for wavefront distortions in-situ providing aberration corrected system. This system is used to enable production of simple holographic optical tweezers apparatus with as many as 50 individual foci to create complex 3D microstructures. These structures can induce the collective behaviour of bacteria.
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Halina Rubinsztein-Dunlop, Declan J. Armstrong, Mark Watson, Patrick Grant, Timo A. Nieminen, Itia Favre-Bulle, Alexander Stilgoe, "Structured light for studies of active matter," Proc. SPIE PC12663, Molecular and Nanophotonic Machines VI, PC1266309 (5 October 2023); https://doi.org/10.1117/12.2678853