Effect of inhibiting PpIX bioconversion and PpIX efflux on 5-aminolevulinic acid-mediated protoporphyrin IX fluorescence and photodynamic therapy in human glioma cell lines

Jordyn Olsen; Bin Chen; Sharayu Chandratre

doi:10.1117/12.3003191

13 March 2024 Effect of inhibiting PpIX bioconversion and PpIX efflux on 5-aminolevulinic acid-mediated protoporphyrin IX fluorescence and photodynamic therapy in human glioma cell lines

Jordyn Olsen, Bin Chen, Sharayu Chandratre

Author Affiliations +

Proceedings Volume PC12823, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXXII; PC1282308 (2024) https://doi.org/10.1117/12.3003191
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Photodynamic therapy (PDT) is a minimally invasive therapy that uses a photosensitizer in combination with light and oxygen to stimulate cell death. ALA-PDT has been approved as an intraoperative imaging agent in patients with high grade glioma. This research investigates the inhibition of PpIX efflux by lapatinib, in combination with the inhibition of PpIX bioconversion by deferoxamine to further increase intracellular PpIX in glioma cell lines. It can be concluded that intracellular PpIX accumulation can be achieved by inhibiting both PpIX efflux and bioconversion leading to cell death in a cell line dependent manner.

Conference Presentation

Citation Download Citation

Jordyn Olsen, Bin Chen, and Sharayu Chandratre "Effect of inhibiting PpIX bioconversion and PpIX efflux on 5-aminolevulinic acid-mediated protoporphyrin IX fluorescence and photodynamic therapy in human glioma cell lines", Proc. SPIE PC12823, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXXII, PC1282308 (13 March 2024); https://doi.org/10.1117/12.3003191

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