High-throughput cell stimulation by femtosecond laser on a lab-on-a-disc (fsL-LOAD)

Zhenming XIE; Shiyue Liu; Wu Yuan; Siu Kai Kong; Aaron Ho-Pui Ho

doi:10.1117/12.3000875

13 March 2024 High-throughput cell stimulation by femtosecond laser on a lab-on-a-disc (fsL-LOAD)

Zhenming XIE, Shiyue Liu, Wu Yuan, Siu Kai Kong, Aaron Ho-Pui Ho

Proceedings Volume PC12840, Optical Interactions with Tissue and Cells XXXV; PC1284008 (2024) https://doi.org/10.1117/12.3000875
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Cellular photomodulation holds promising potential in biomedical research, However, this technique is typically performed manually at low speed. We have successfully developed an integrated femtosecond laser (fsL) cell stimulation system based on a constant radius lab-on-a-disc (LOAD) platform in which a concentric microchannel for rearranging cells to a monolayer under centrifugal force can be engraved in a polymethyl methacrylate layer in situ while the disc is in spinning mode. The system can perform multi-photon cell stimulation, which subsequently leads to molecular signaling modulation of cells in a high-throughput and highly automatic manner.

This project was supported by General Research Fund (GRF) : 14204621, 14207920 and 14207419.

Conference Presentation

Citation Download Citation

Zhenming XIE, Shiyue Liu, Wu Yuan, Siu Kai Kong, and Aaron Ho-Pui Ho "High-throughput cell stimulation by femtosecond laser on a lab-on-a-disc (fsL-LOAD)", Proc. SPIE PC12840, Optical Interactions with Tissue and Cells XXXV, PC1284008 (13 March 2024); https://doi.org/10.1117/12.3000875

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KEYWORDS

Femtosecond phenomena

Calcium

Modulation

Stem cells

Laboratories

Laser applications

Laser development

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