Time-gated approach for lens-free fluorescence microscopy

Kenneth Lang; Maryam Baker; Euan McLeod

doi:10.1117/12.3003088

13 March 2024 Time-gated approach for lens-free fluorescence microscopy

Kenneth Lang, Maryam Baker, Euan McLeod

Proceedings Volume PC12857, Computational Optical Imaging and Artificial Intelligence in Biomedical Sciences; PC128570J (2024) https://doi.org/10.1117/12.3003088
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Fluorescent nano-particle labeling methods produce high contrast signals for rare-cell detection. Unfortunately, many imaging methods cannot yet reach adequate space-bandwidth products for these samples. We propose a lens-free time-gated fluorescence system using pulsed excitation and long-lifetime fluorescent nanoparticles. We minimize the required sample to sensor distance by applying a temporal filter instead of spectral filters and achieve a resolution of 8.77 μm. This approach simplifies the architecture, requires minimal image reconstruction, and reduces system size and cost. Our method surpasses the performance of other non-computational fluorescent lens-free imaging approaches and provides a foundation for future resolution enhancement.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Kenneth Lang, Maryam Baker, and Euan McLeod "Time-gated approach for lens-free fluorescence microscopy", Proc. SPIE PC12857, Computational Optical Imaging and Artificial Intelligence in Biomedical Sciences, PC128570J (13 March 2024); https://doi.org/10.1117/12.3003088

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