Lateral-flow immunoassay using high aspect ratio plasmonic gold nanostars

Supriya Atta; Tuan Vo-Dinh

doi:10.1117/12.3003588

13 March 2024 Lateral-flow immunoassay using high aspect ratio plasmonic gold nanostars

Supriya Atta, Tuan Vo-Dinh

Author Affiliations +

Proceedings Volume PC12860, Plasmonics in Biology and Medicine XXI; PC1286006 (2024) https://doi.org/10.1117/12.3003588
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

To improve the LFIA sensitivity, we have utilized high aspect-ratio plasmonic gold nanostars (GNS), which possess higher optical brightness than traditional gold nanospheres. We will discuss here the surfactant-free GNS synthesis in which the aspect ratio of the GNS can be tuned by changing the concentration of three reagents, ascorbic acid, silver nitrate (AgNO3), and hydrochloric acid (HCl). Herein, we selected the bacterium Yersinia pestis as a model analyte system, and the LFIA efficiency was investigated with the optical density measurements of the test line by utilizing different aspect-ratio GNSs. Our results showed that the maximum LFIA sensitivity was achieved with the GNS morphology having the maximum aspect-ratio spikes. Compared to other nanoparticle-based LFIA systems, high aspect-ratio GNS exhibits high analytical sensitivity, indicating it to be a promising candidate to become a much more versatile and tunable LFIA sensor.

Conference Presentation

Citation Download Citation

Supriya Atta and Tuan Vo-Dinh "Lateral-flow immunoassay using high aspect ratio plasmonic gold nanostars", Proc. SPIE PC12860, Plasmonics in Biology and Medicine XXI, PC1286006 (13 March 2024); https://doi.org/10.1117/12.3003588

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