Since the publication of the first edition of the Handbook in 2002, optical methods for biomedical diagnostics have developed in many well-established directions, and new trends have also appeared. To encompass all current methods, the text has been updated and expanded into two volumes.

Volume 2: Methods begins by describing the basic principles and diagnostic applications of optical techniques based on detecting and processing the scattering, fluorescence, FT IR, and Raman spectroscopic signals from various tissues, with an emphasis on blood, epithelial tissues, and human skin. The second half of the volume discusses specific imaging technologies, such as Doppler, laser speckle, optical coherence tomography (OCT), and fluorescence and photoacoustic imaging.