Surface plasmon resonance phase-shift interferometry: Real-time DNA microarray hybridization analysis

Shean-Jen Chen; Yuan-Deng Su; Feng-Ming Hsiu; Chia-Yuan Tsou; Yi-Kuang Chen

doi:10.1117/1.1924713

1 May 2005 Surface plasmon resonance phase-shift interferometry: Real-time DNA microarray hybridization analysis

Shean-Jen Chen, Yuan-Deng Su, Feng-Ming Hsiu, Chia-Yuan Tsou, Yi-Kuang Chen

Author Affiliations +

Journal of Biomedical Optics, Vol. 10, Issue 3, 034005 (May 2005). https://doi.org/10.1117/1.1924713

Abstract

Surface plasmon resonance (SPR) phase-shift interferometry (PSI) is a novel technique which combines SPR and modified Mach–Zehnder PSI to measure the spatial phase variation caused by biomolecular interactions upon a sensing chip. The SPR-PSI imaging system offers high resolution and high-throughout screening capabilities for microarray DNA hybridization without the need for additional labeling, and provides valuable quantitative information. The SPR-PSI imaging system has an enhanced detection limit of 2.5×10^–7 refraction index change, a long-term phase stability of π/100 in 30 min, and a spatial phase resolution of π/300 with 100×100 µm² detection area. This study successfully demonstrates the label-free observation of 15-mer DNA microarray.

©(2005) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Shean-Jen Chen, Yuan-Deng Su, Feng-Ming Hsiu, Chia-Yuan Tsou, and Yi-Kuang Chen "Surface plasmon resonance phase-shift interferometry: Real-time DNA microarray hybridization analysis," Journal of Biomedical Optics 10(3), 034005 (1 May 2005). https://doi.org/10.1117/1.1924713

Published: 1 May 2005

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KEYWORDS

Imaging systems

Surface plasmons

Phase measurement

Reconstruction algorithms

Gold

Refractive index

Biological research

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