Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer

Kristen E. Adams; Shi Ke; Sunkuk Kwon; Feng Liang; Zhen Fan; Yang Lu; Karen Hirshi; Michel E. Mawad; Michael A. Barry; Eva Marie Sevick-Muraca

doi:10.1117/1.2717137

1 March 2007 Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer

Kristen E. Adams, Shi Ke, Sunkuk Kwon, Feng Liang, Zhen Fan, Yang Lu, Karen Hirshi, Michel E. Mawad, Michael A. Barry, Eva Marie Sevick-Muraca

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Journal of Biomedical Optics, Vol. 12, Issue 2, 024017 (March 2007). https://doi.org/10.1117/1.2717137

Abstract

Targeted fluorescent molecular imaging probes may provide an optimal means of detecting disease. Stable, organic fluorophores can be repeatedly excited in vivo by propagated light and consequentially can provide large signal-to-noise ratios (SNRs) for image detection of target tissues. In the literature, many small animal imaging studies are performed with a red excitable dye, Cy5.5, conjugated to the targeting component. We report the comparison of the in vivo fluorescent imaging performance of a near-IR (NIR) and a red-excitable dye. Epidermal growth factor (EGF) was conjugated with Cy5.5 [excitation/emission (ex/em), 660/710 nm] or IRDye® 800CW (ex/em: 785/830 nm) for imaging EGF receptor (EGFr) positive (MDA-MB-468) and/or negative (MDA-MB-435) human breast cancer cell lines in subcutaneous xenograft models. The conjugates were injected intravenously at 1-nmol-dye equivalent with and without anti-EGFr monoclonal antibody C225, preadministered 24 h prior as a competitive ligand to EGFr. Our images show that while both agents target EGFr, the EGF-IRDye® 800CW evidenced a significantly reduced background and enhanced the tumor-to-background ratio (TBR) compared to the EGF-Cy5.5. Immunohistochemistry shows that EGF causes activation of the EGFr signaling pathway, suggesting that prior to use as a targeting, diagnostic agent, potential deleterious effects should be considered.

©(2007) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Kristen E. Adams, Shi Ke, Sunkuk Kwon, Feng Liang, Zhen Fan, Yang Lu, Karen Hirshi, Michel E. Mawad, Michael A. Barry, and Eva Marie Sevick-Muraca "Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer," Journal of Biomedical Optics 12(2), 024017 (1 March 2007). https://doi.org/10.1117/1.2717137

Published: 1 March 2007

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