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1 September 2010 Identification of source of calcium in HeLa cells by femtosecond laser excitation
Hao He, Siu-Kai Kong, Kam Tai Chan
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Journal of Biomedical Optics, Vol. 15, Issue 5, 057010 (September 2010). https://doi.org/10.1117/1.3485741
Abstract
Calcium is an important messenger in cells and whose store and diffusion dynamics at the subcellular level remain unclear. By inducing a controlled slow subcellular Ca2+ release through femtosecond laser irradiation in HeLa cells immersed in different media, cytoplasm is identified to be the major intracellular Ca2+ store, with the nucleus being the minor store and the extracellular Ca2+ also contributing to the total cellular Ca2+ level. Furthermore, Ca2+ released in either the cytoplasm or nucleus diffuses into the nucleus or cytoplasm, respectively, at different rates and influences the Ca2+ release in those regions.
©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Hao He, Siu-Kai Kong, and Kam Tai Chan "Identification of source of calcium in HeLa cells by femtosecond laser excitation," Journal of Biomedical Optics 15(5), 057010 (1 September 2010). https://doi.org/10.1117/1.3485741
Published: 1 September 2010
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Cited by 11 scholarly publications.
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KEYWORDS
Calcium
Femtosecond phenomena
Luminescence
Diffusion
Confocal microscopy
Electronics engineering
Microscopes
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