Investigation of the hyper-reflective inner/outer segment band in optical coherence tomography of living frog retina

Rong-Wen Lu; Qiu-Xiang Zhang; Xincheng Yao; Christine A. Curcio; Youwen Zhang; Steven Pittler; Dusanka Deretic

doi:10.1117/1.JBO.17.6.060504

6 June 2012 Investigation of the hyper-reflective inner/outer segment band in optical coherence tomography of living frog retina

Rong-Wen Lu, Qiu-Xiang Zhang, Xincheng Yao, Christine A. Curcio, Youwen Zhang, Steven Pittler, Dusanka Deretic

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Journal of Biomedical Optics, Vol. 17, Issue 6, 060504 (June 2012). https://doi.org/10.1117/1.JBO.17.6.060504

Abstract

This study is to test anatomic correlates, including connecting cilium (CC) and inner segment (IS) ellipsoid, to the hyper-reflective band visualized by optical coherence tomography (OCT) and commonly attributed to the photoreceptor inner/outer segment (IS/OS) junction. A line-scan OCT (LS-OCT) was constructed to achieve sub-cellular resolution (lateral: ~ 2 μm; axial: ~ 4 μm) of excised living frog retinas. An electro-optic phase modulator was employed for rapid and vibration-free phase modulation. Comparison of normalized distance measurements between LS-OCT images and histological images revealed that the dominant source of the signal reported as the IS/OS OCT band actually originates from the IS.

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Rong-Wen Lu, Qiu-Xiang Zhang, Xincheng Yao, Christine A. Curcio, Youwen Zhang, Steven Pittler, and Dusanka Deretic "Investigation of the hyper-reflective inner/outer segment band in optical coherence tomography of living frog retina," Journal of Biomedical Optics 17(6), 060504 (6 June 2012). https://doi.org/10.1117/1.JBO.17.6.060504

Published: 6 June 2012

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