Measuring the dynamics of cyclic adenosine monophosphate level in living cells induced by low-level laser irradiation using bioluminescence resonance energy transfer

Yimei Huang; Liqin Zheng; Hongqin Yang; Jiangxu Chen; Yuhua Wang; Hui Li; Shusen Xie; Haishan Zeng

doi:10.1117/1.JBO.20.5.051029

22 January 2015 Measuring the dynamics of cyclic adenosine monophosphate level in living cells induced by low-level laser irradiation using bioluminescence resonance energy transfer

Yimei Huang, Liqin Zheng, Hongqin Yang, Jiangxu Chen, Yuhua Wang, Hui Li, Shusen Xie, Haishan Zeng

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Journal of Biomedical Optics, Vol. 20, Issue 5, 051029 (January 2015). https://doi.org/10.1117/1.JBO.20.5.051029

Abstract

Several studies demonstrated that the cyclic adenosine monophosphate (cAMP), an important second messenger, is involved in the mechanism of low-level laser irradiation (LLLI) treatment. However, most of these studies obtained the cAMP level in cell culture extracts or supernatant. In this study, the cAMP level in living cells was measured with bioluminescence resonance energy transfer (BRET). The effect of LLLI on cAMP level in living cells with adenosine receptors blocked was explored to identify the role of adenosine receptors in LLLI. The results showed that LLLI increased the cAMP level. Moreover, the rise of cAMP level was light dose dependent but wavelength independent for 658-, 785-, and 830-nm laser light. The results also exhibited that the adenosine receptors, a class of G protein-coupled receptor (GPCR), modulated the increase of cAMP level induced by LLLI. The cAMP level increased more significantly when the A₃ adenosine receptors (A₃R) were blocked by A₃R antagonist compared with A₁ adenosine receptor or A_2a adenosine receptor blocked in HEK293T cells after LLLI, which was in good agreement with the adenosine receptors’ expressions. All these results suggested that measuring the cAMP level with BRET could be a useful technique to study the role of GPCRs in living cells under LLLI.

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Yimei Huang, Liqin Zheng, Hongqin Yang, Jiangxu Chen, Yuhua Wang, Hui Li, Shusen Xie, and Haishan Zeng "Measuring the dynamics of cyclic adenosine monophosphate level in living cells induced by low-level laser irradiation using bioluminescence resonance energy transfer," Journal of Biomedical Optics 20(5), 051029 (22 January 2015). https://doi.org/10.1117/1.JBO.20.5.051029

Published: 22 January 2015

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