Terahertz (THz) technology has become popular worldwide as a new approach to detecting biomolecules because the vibrational and rotational energy levels of many biomolecules fall in the THz band and because the THz wave has the characteristics of low electronic energy, which will not damage the samples to be measured. Many biomolecules need to maintain their biological activity in liquid environment. However, as a polar molecule, water has a strong absorption of THz wave, which is mainly because the vibration frequency of hydrogen bond in aqueous solution is within the THz frequency range. Therefore, the best solution is to reduce the action distance between the aqueous solution and THz wave and control it within 100  μm. Microfluidic chips can meet such requirements. Therefore, the combination of THz technology and microfluidic technology can study the dynamic characteristics of biomolecules in an aqueous solution. The microfluidic chip was fabricated using ZEONOR 1420Rs. The THz transmittance of the material can exceed 95%. The depth of the microchannel in the microfluidic chip is 50  μm. In addition, the chip has the characteristics of good airtightness, portability, convenient disassembling, and reusability. Seventeen kinds of electrolytes were tested with the chip. The results show that the THz spectral intensity of electrolyte composed of different anions and cations, so the spectral characteristics of other electrolyte solutions can be obtained according to the spectral information of these detected ions.