Speckle pattern texture analysis method is applied to measure surface roughness of human skin. The method is based on analyzing of a gray level co-occurrence matrix occurred from a speckle image of a rough surface. Paper with different surface roughness is used as a skin phantom. The roughness is controlled by profilometry measurements. The developed methodology could find wide application in dermatology and tissue diagnostics.
Oxidative stress results from an imbalance between the production and detoxification of reactive oxygen spices (ROS).
ROS are natural byproducts of normal metabolism of oxygen and have important roles in cell signaling and homeostasis.
Many heart related diseases like heart failure and myocardial infarction develop as a result of oxidative stress. Current
treatment cannot improve the progressive decline in heart function experienced by all patients. Therefore heart failure is
the cause of around 25% of all deaths in the Asia Pacific region. Thus any step taken to address the oxidative stress
problem is essential for enhancing human health and improve their quality of life. Current approach is dedicated to
develop micron-size oxidation stress-sensor for in-vivo measuring level of ROS in KillerRed expressing transgenic
zebrafish larvae. Central to our investigation is the light-inducible heart failure animal model we developed in zebrafish
that expressed KillerRed in the heart. By utilizing the photosensitizer properties of KillerRed to produce ROS upon green
light illumination, heart failure can be repeatedly induced in a non-invasive manner. Importantly, the use of this
biological platform permits the development of physiologically sensitive ROS sensor and identifies efficient antioxidants
that improve heart contractility. The biosensor approach is based on utilizing biocompatible polyelectrolyte
microcapsules as a carry of fluorescent dyes sensitive to amount of reactive oxygen spices. Microcapsule prevents dye
diffusion in tissue that makes use toxic dyes possible. Microcapsule's wall is permeable for environment with size less
than 500 Da. The oxidation stress-sensors are injected directly in zebrafish pericardium with further circulation along
blood system. Detecting of ROS is obtained by using laser scanning microscopy by illuminating oxidation stress-sensors
and detecting changing excitation signal from the fluorescent dye.
Analysis of images of moving liquid-gas boundaries in porous samples (sheets of paper) under white light and coherent
illumination was used to study the scaling properties of growing interfaces near the pinning condition. The obtained
images were digitized and processed in order to obtain the local and global parameters of interface mobility at different
stages of interface growth. At early stage of growth, the interface dynamics is characterized by the familiar Lucas-Washburn (LW) equation, which describes continuous-like motion of interface. At the late stage of growth, near the
pinning condition, the interface motion exhibits the deviation from LW model, which is the result of pronounced discrete
character of interface evolution. The developed technique of imbibition analysis can be applied to structural study of
porous media of various nature.
The Langmuir-Blodgett technique has been applied to build optically homogeneous monolayer films of multi-wall carbon nanotubes (MWNTs) which possess good surface spreading properties at the air/water interface. Mono-layers of MWCNTs- arahidic acid were deposited on glass substrates with ITO contacts by vertical lifting. Prepared substrates used to make liquid crystals optical cells to detecting morphology of MWCNT mono-layers which play the aligning coveting role. We demonstrate that MWCNT aligned by one direction in the Langmuir-Blodgett film which was transferred on the substrate with keeping MWCNTs aligning.
We have experimentally investigated for the first time the electro-optical response of polymer dispersed liquid crystals (PDLC) doped with multi-wall carbon nanotubes (MWCNT). Undoped PDLC and PDLC doped with MWCNT were studied. Principal transmittances of the films and angular distributions of scattered light were measured as the functions of applied voltage. Anomalous electro-optical response of PDLC doped with MWCN was observed with cross-polarized light. Carbon nanotubes influence on the droplet director orientation in PDLC was found. The obtained results are discussed in terms of the model which takes into account the partial orientation of liquid crystal droplets in PVA matrix together with the presence of carbon nanotubes.
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