A new azimuthally stable polarimetric method for processing of microscopic images of optically anisotropic structures of different biological layers blood plasma is proposed. A new model of phase anisotropy definition of biological tissues by using superposition of Mueller matrices of linear birefringence and optical activity is proposed. The matrix element M44 has been chosen as the main information parameter, which value is independent of rotation angle of both sample and probing beam polarization plane.
A new information optical technique of diagnostics of the structure of the polycrystalline bile films is proposed. The model of Mueller-matrix description of mechanisms of optical anisotropy of such objects as optical activity, birefringence, as well as linear and circular dichroism is suggested. The ensemble of informationally topical azimuthally stable Mueller-matrix invariants is determined. Within the statistical analysis of such parameters distributions the objective criteria of differentiation of the polycrystalline bile films taken from patients with fatty degeneration (group 1) chronic hepatitis (group 2) of the liver were determined. From the point of view of probative medicine the operational characteristics (sensitivity, specificity and accuracy) of the information-optical method of Mueller-matrix mapping of polycrystalline films of bile were found and its efficiency in diagnostics of pathological changes was demonstrated.
The work consists of investigation results of diagnostic efficiency of a new azimuthally stable Muellermatrix method of analysis of laser autofluorescence coordinate distributions of biological tissues histological sections. A new model of generalized optical anisotropy of biological tissues protein networks is proposed in order to define the processes of laser autofluorescence. The influence of complex mechanisms of both phase anisotropy (linear birefringence and optical activity) and linear (circular) dichroism is taken into account. The interconnections between the azimuthally stable Mueller-matrix elements characterizing laser autofluorescence and different mechanisms of optical anisotropy are determined. The statistic analysis of coordinate distributions of such Mueller-matrix rotation invariants is proposed. Thereupon the quantitative criteria (statistic moments of the 1st to the 4th order) of differentiation of histological sections of myometrium – group 1 (fibromyoma) and group 2 (adenocarcinoma) are estimated.
A new azimuthally stable polarimetric method for processing of microscopic images of optically anisotropic structures of different biological layers histological sections is proposed. A new model of phase anisotropy definition of biological tissues by using superposition of Mueller matrices of linear birefringence and optical activity is proposed. The matrix element M44 has been chosen as the main information parameter, which value is independent of rotation angle of both sample and probing beam polarization plane.
The theoretical background of azimuthally stable method Jones matrix mapping of histological sections of biopsy
of uterine neck on the basis of spatial-frequency selection of the mechanisms of linear and circular birefringence is
presented. The comparative results of measuring the coordinate distributions of complex degree of mutual anisotropy
formed by fibrillar networks of myosin and collagen fibrils of uterine neck tissue of different pathological states - precancer
(dysplasia) and cancer (adenocarcinoma) are shown. The values and ranges of change of the statistical (moments
of the 1st - 4th order) parameters of complex degree of mutual anisotropy coordinate distributions are studied. The
objective criteria of diagnostics of the pathology and differentiation of its severity degree are determined.
To analyze the coordinate-like structure of Stokes-parametric and Mueller-matrix images of optically anisotropic components of biological tissues, the two-point polarization-correlational approach was applied. On this basis parameters the method of cross-correlational definition of parameters (average sizes, asymmetry factor) of correlational contour was developed, which defines the topographical structure of the characteristic meanings of Stokes-parametric and Mueller-matrix images of histological sections of biological tissues.
It has been proposed an optical model of linear and circular birefringence polycrystalline structure of biological crystallites. Method of polarizing optical mapping of anisotropic polycrystalline networks with spatial frequency filtering azimuth coordinate distributions of laser radiation polarization in Fourier- plane is analytically proved. Also was made an analytical study of the effectiveness of the method spatial-frequency selection in differentiation distributions azimuth field of laser radiation.
This research is aimed to investigate the reliability of Mueller-matrix differentiation of birefringence
change of optically thick layers of biological tissues at the early stages of the change in their physiological
state. This is performed by measuring the set of asymmetry and excess values of Mueller matrix image of the
phase element M44 in various points of the object under investigation.
The model of laser polarization fluorescence of biological tissues considering the mechanisms of
optically anisotropic absorption – linear and circular dichroism of protein networks was suggested.Muellermatrix
rotation invariants characterizing polarization manifestations of laser fluorescence are determined.The
interconnections between the statistical, correlation and fractal parameters characterizing the Mueller-matrix
images of laser polarization fluorescence and the peculiarities of the mechanisms of optically anisotropic
absorption of histological sections of uterus wall biopsy were found. Effectiveness of the method of azimuthinvariant
Mueller-matrix mapping of laser polarization fluorescence of protein networks in the task of
differentiation of benign and malignant tumors of uterus wall was demonstrated.
The complex technique of the concerted polarization-phase and spatial-frequency filtering of blood plasma laser images
has been suggested. The possibility of obtaining separately the coordinate distributions of phases of linearly and
circularly birefringent protein networks of blood plasma has been presented. The diagnostically sensitive parameters of a
pathological change of the birefringence of blood plasma polycrystalline networks were determined. The effectiveness of
the developed technique for detecting the change of birefringence of the blood plasma smears in diagnostics and
differentiation of the acute and gangrenous appendicitis (exudate) is shown.
This paper presented data on the method of coherent space-frequency filtering of coordinate distributions
azimuth and ellipticity of polarization of the laser plasma image. The optical model of polycrystalline networks of human
blood plasma is suggested. The results of investigating the interrelation between the values of blood plasma polarization
maps statistical (statistical moments of the 1st-4th order) parameters and pathological changes of rectum wall are
presented. They characterize the coordinate distributions of the azimuth and ellipticity of Fourier transforms of laser
images of human blood plasma. The diagnostic criteria of rectum cancer are determined.
The paper deals with investigating the processes of laser radiation transformation by biological crystals networks using the singular optics techniques. The results obtained showed a distinct correlation between the points of "characteristic" values of coordinate distributions of Mueller matrix elements and polarization singularities (S- and C-points) of laser transformation of biological crystals networks with the following possibility of Mueller-matrix selection of polarization singularity. The technique of Mueller-matrix singular diagnostics of pathological changes of women's reproductive sphere tissue (myometrium) is proposed.
The possibilities of the local wavelet-analysis of polarization-inhomogeneous laser image of human blood plasma were
considered. The set of statistics, correlation and fractal parameters of the distributions of wavelet-coefficients that are
characterize different scales of the polarization maps of polycrystalline networks of amino acids of blood plasma were
defined. The criteria for the differentiation of the transformation of birefringence optical-anisotropic structures of blood
plasma at different scales of their geometric dimensions were determined.
The interrelations between statistics of the 1st-4th orders of the ensemble of Mueller-matrix images and geometric
structure of birefringent architectonic nets of different morphological structure have been analyzed. The sensitivity of
asymmetry and excess of statistic distributions of matrix elements cik to changing of orientation structure of optically
anisotropic protein fibrils of physiologically normal and pathologically changed biological tissues architectonics has
been shown.
The paper is aimed at researching the effectiveness of differentiation of optical anisotropic property of
biological crystal networks by means of the statistic and correlation analysis of new analytical parameter -
the degree of mutual correlation of biological tissues Mueller matrices of different morphological structure
and physiological state.
To characterize the degree of consistency of parameters of the optically uniaxial birefringent protein nets of blood
plasma a new parameter - complex degree of mutual anisotropy is suggested. The technique of polarization measuring
the coordinate distributions of the complex degree of mutual anisotropy of blood plasma is developed. It is shown that
statistic approach to the analysis of complex degree of mutual anisotropy distributions of blood plasma is effective in the
diagnosis and differentiation of acute inflammation - acute and gangrenous appendicitis.
In this work, we have theoretically grounded conceptions of characteristics points observed in coordinate
distributions of Mueller matrix elements for a network of human tissue biological crystals. Found is
interrelation between polarization singularities of laser images inherent to these biological crystals and
characteristic values of above matrix elements. We have determined criteria for statistical diagnostics of
pathological changes in the birefringent structure of biological crystal network by using myometrium tissue as
an example.
To characterize the degree of consistency of parameters of the optically uniaxial birefringent protein nets of blood
plasma a new parameter - complex degree of mutual anisotropy is suggested. The technique of polarization measuring
the coordinate distributions of the complex degree of mutual anisotropy of blood plasma is developed. It is shown that
statistic approach to the analysis of complex degree of mutual anisotropy distributions of blood plasma is effective in the
diagnosis and differentiation of acute inflammation - acute and gangrenous appendicitis.
To characterize the degree of consistency of parameters of the optically uniaxial
birefringent protein fibrils nets of biological tissues a new parameter - complex degree of
mutual anisotropy is suggested. The technique of polarization measuring the coordinate
distributions of the complex degree of mutual anisotropy of biological tissues is developed. It
is shown that statistic approach to the analysis of complex degree of mutual anisotropy
distributions of skin derma of various optical thicknesses appears to be more sensitive and
efficient in differentiation of physiological state in comparison with investigations of complex
degree of mutual polarization of the corresponding laser images.
The possibilities of local wavelet analysis of phase inhomogeneous laser images of human blood plasma have been
considered in this work. It has been defined the set of statistic, correlation and fractal parameters of wavelet coefficient
distributions, which characterize the different scales of polarization maps of blood plasma. Also, the discrimination
criteria of the birefringence transformation processes of blood plasma anisotropic structures on different geometric scales
have been estimated.
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