The design of high-NA optical systems such as those used for advanced single molecule microscopy require careful modeling of polarization and its impact on the point spread function. When the system is engineered specifically with a polarization-dependent point spread function, it is possible to recover information such as molecule location, orientation, and wobble. SOLID comprises five principles of software design that, when implemented in software designed for modeling a microscope, can provide significant flexibility in the shape of a fluorescent source and its polarization distribution while incorporating such novel elements as stress-engineered optics in the imaging path. We will describe a modeling architecture implemented in Python and provide examples relevant to experimental single molecule microscopy systems.
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