Mr. Dean C. S. Tai Profile

Dean C. S. Tai

Ph.D. Student at Institute of Bioengineering and Nanotechnology

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Author

Publications (11)

SPIE Journal Paper | 1 November 2011 Open Access

Assessment of liver steatosis and fibrosis in rats using integrated coherent anti-Stokes Raman scattering and multiphoton imaging technique

Jian Lin, Fake Lu, Wei Zheng, Zhiwei Huang, Shuoyu Xu, Dean Tai, Hanry Yu

JBO, Vol. 16, Issue 11, 116024, (November 2011) https://doi.org/10.1117/12.10.1117/1.3655353

KEYWORDS: Liver, Tissues, Second-harmonic generation, Collagen, Microscopy, CARS tomography, Tissue optics, Imaging systems, 3D image processing, Surgery

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Proceedings Article | 23 February 2011 Paper

Multimodal nonlinear optical imaging of obesity-induced liver steatosis and fibrosis

Jian Lin, Fake Lu, Wei Zheng, Dean C. Tai, Hanry Yu, Colin Sheppard, Zhiwei Huang

Proceedings Volume 7903, 79031V (2011) https://doi.org/10.1117/12.873507

KEYWORDS: Liver, Tissues, Microscopy, Collagen, Second-harmonic generation, Optical microscopy, CARS tomography, Multimodal imaging, Nonlinear optics, Optical imaging

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SPIE Journal Paper | 1 September 2010 Open Access

Pulse-modulated second harmonic imaging microscope quantitatively demonstrates marked increase of collagen in tumor after chemotherapy

Anju Raja, Shuoyu Xu, Wanxin Sun, Jianbiao Zhou, Dean Tai, Chien-Shing Chen, Jagath Rajapakse, Peter So, Hanry Yu

JBO, Vol. 15, Issue 05, 056016, (September 2010) https://doi.org/10.1117/12.10.1117/1.3497565

KEYWORDS: Collagen, Tumors, Scanning helium ion microscopy, Microscopes, Second-harmonic generation, Signal to noise ratio, Visualization, Tissues, Modulation, Bragg cells

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SPIE Journal Paper | 1 September 2010 Open Access

Toward surface quantification of liver fibrosis progression

Yuting He, Chiang Huen Kang, Shuoyu Xu, Xiaoye Tuo, Scott Trasti, Dean Tai, Anju Raja, Qiwen Peng, Peter So, Jagath Rajapakse, Roy Welsch, Hanry Yu

JBO, Vol. 15, Issue 05, 056007, (September 2010) https://doi.org/10.1117/12.10.1117/1.3490414

KEYWORDS: Liver, Collagen, Natural surfaces, Tissues, Second-harmonic generation, Biopsy, Image segmentation, Feature extraction, Nanotechnology, Binary data

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Proceedings Article | 26 February 2010 Paper

Assessment of fibrotic liver disease with multimodal nonlinear optical microscopy

Fake Lu, Wei Zheng, Dean C. S. Tai, Jian Lin, Hanry Yu, Zhiwei Huang

Proceedings Volume 7569, 75691W (2010) https://doi.org/10.1117/12.843127

KEYWORDS: Liver, Collagen, Microscopy, Tissues, Optical microscopy, Second-harmonic generation, CARS tomography, Optical fibers, Medical research, Multiphoton microscopy

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Proceedings Article | 24 February 2010 Paper

Time-resolved all fiber fluorescence spectroscopy system

A. Chen, F. Vanholsbeeck, D. Tai, M. Svrcek, B. Smaill

Proceedings Volume 7559, 755905 (2010) https://doi.org/10.1117/12.840811

KEYWORDS: Luminescence, Heart, Spectroscopy, Signal to noise ratio, Action potentials, Fluorescence spectroscopy, Data acquisition, Fiber optics, Optical filters, Optical fibers

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SPIE Journal Paper | 1 July 2009 Open Access

Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy

Dean Tai, Nancy Tan, Shuoyu Xu, Chiang Huen Kang, Ser Mien Chia, Chee Leong Cheng, Aileen Wee, Chiang Li Wei, Anju Raja, Guangfa Xiao, Shi Chang, Jagath Rajapakse, Peter So, Hui-Huan Tang, Chien Shing Chen, Hanary Yu

JBO, Vol. 14, Issue 04, 044013, (July 2009) https://doi.org/10.1117/12.10.1117/1.3183811

KEYWORDS: Liver, Collagen, Second-harmonic generation, Tissues, Microscopy, Image segmentation, Algorithm development, Tissue optics, Harmonic generation, Animal model studies

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SPIE Journal Paper | 1 November 2008 Open Access

Nonlinear optical microscopy: use of second harmonic generation and two-photon microscopy for automated quantitative liver fibrosis studies

Wanxin Sun, Shi Chang, Dean Tai, Nancy Tan, Guangfa Xiao, Huihuan Tang, Hanry Yu

JBO, Vol. 13, Issue 06, 064010, (November 2008) https://doi.org/10.1117/12.10.1117/1.3041159

KEYWORDS: Collagen, Liver, Second-harmonic generation, Tissues, Microscopy, Optical fibers, Imaging systems, Optical microscopy, Harmonic generation, Tissue optics

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Proceedings Article | 16 May 2008 Paper

Time-resolved all fiber fluorescence spectroscopy system

A. Y. Chen, F. Vanholsbeeck, D. C. Tai, S. Swift, N. Singhal, J. Harvey, D. Hooks, B. Smaill

Proceedings Volume 7004, 70042G (2008) https://doi.org/10.1117/12.785967

KEYWORDS: Luminescence, Action potentials, Spectroscopy, Bacteria, Optical filters, Fluorescence spectroscopy, Heart, Mirrors, Fiber optics, Green fluorescent protein

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SPIE Journal Paper | 1 May 2007 Open Access

Illumination and fluorescence collection volumes for fiber optic probes in tissue

Dean Tai, Darren Hooks, John Harvey, Bruce Smaill, Christian Soeller

JBO, Vol. 12, Issue 03, 034033, (May 2007) https://doi.org/10.1117/12.10.1117/1.2750288

KEYWORDS: Luminescence, Scattering, Tissue optics, Optical fibers, Light scattering, Photolysis, Absorption, Tissues, Fiber optics, Data modeling

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Proceedings Article | 4 April 2005 Paper

A modular fiber optic system for intramural functional fluorescence measurement

Dean Tai, Sally Rutherford, Bryan Caldwell, Ian LeGrice, John Harvey, Bruce Smaill

Proceedings Volume 5704, (2005) https://doi.org/10.1117/12.584147

KEYWORDS: Luminescence, Imaging systems, Action potentials, Fiber optics, Heart, Biomedical optics, Solid state lasers, Potassium, Light sources, Optical fibers

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Fluorescence imaging techniques have been central to much biomedical science research over the past two decades. In particular, functional imaging has provided important new information about processes that occur at cellular and sub-cellular levels. With this approach, living tissues are stained with dyes whose emission is modulated by changes in the environment to which the dye is exposed. The fluorescence imaging systems used within this context typically incorporate relatively complex free space optical assemblies and a stable platform is necessary to maintain appropriate alignment of their components. Because of the poor efficiency of these systems, it is necessary to use powerful light sources and sensitive photo-detectors. We have developed a novel fluorescence imaging system in which free-space optics are replaced by optical fibers, passive optical splitters and associated components. Solid state lasers are used as the excitation light source. A variety of detection systems have been utilized including a spectrometer. The feasibility of the approach has been established using a rat heart preparation stained with the membrane potential-sensitive dye, di-4-ANEPPS. Detailed emission spectra for this dye, at different levels of resting membrane potential, are presented here for 532 nm and 488 nm excitation. Cardiac action potentials obtained with the modular fiber optic system correspond closely to intracellular potentials acquired at adjacent sites in the isolated rat heart preparation. Our modular fiber optic system is cheaper, more efficient, more flexible and more robust than conventional fluorescence imaging systems. Using a high-speed spectrometer for photodetection, it is possible to implement the signal processing required for multi-line or ratiometric imaging in software, which further enhances the efficiency and flexibility of the system. We believe that this approach has wide potential applications for biomedical fluorescence imaging.

Showing 5 of 11 publications

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