Dr. Frank Sonntag Profile

Dr. Frank Sonntag

Group Manager Micro- and Biosystems Engineering at Fraunhofer IWS

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Author

Publications (18)

Proceedings Article | 4 March 2022 Presentation + Paper

Enhanced fluorescence detection of miRNAs using one-dimensional photonic crystal-based biochips

Tommaso Pileri, Alberto Sinibaldi, Norbert Danz, Giuseppe Pignataro, Peter Munzert, Agostino Occhicone, F. Sonntag, Francesco Michelotti

Proceedings Volume 11979, 1197909 (2022) https://doi.org/10.1117/12.2623479

KEYWORDS: Luminescence, Photonic crystals, Fluorescence spectroscopy, Microfluidics, Crystals, Plasma, Molecules, Biomedical optics, Target detection, Liquids

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Proceedings Article | 4 March 2022 Presentation

Multiplexed bio-assay for a reliable detection of HER2 in human plasma using a combined label-free/fluorescence biosensing platform

Tommaso Pileri, Alberto Sinibaldi, Matteo Allegretti, Norbert Danz, Peter Munzert, Elena Giordani, Agostino Occhicone, Frank Sonntag, Patrizio Giacomini, Francesco Michelotti

Proceedings Volume PC11979, PC1197903 (2022) https://doi.org/10.1117/12.2623466

KEYWORDS: Plasma, Biosensing, Multiplexing, Oncology, Cancer, Breast cancer, Refractive index, Reflectivity, Receptors, Proteins

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Proceedings Article | 2 March 2022 Presentation + Paper

New joining and automation approaches for multilayer-based manufacturing of complex, customized microsystems

Stephan Behrens, Thomas Himmer, Uwe Kühsel, Florian Schmieder, Daniel Klaue, Katja Günther, Frank Sonntag

Proceedings Volume 11955, 119550B (2022) https://doi.org/10.1117/12.2625455

KEYWORDS: Plasma treatment, Manufacturing, Microfluidics, Microsystems, Polymers, Computer aided design, System integration, Robotic systems, Microscopes, Medicine

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Proceedings Article | 16 April 2021 Presentation

SEPSIS biomarker detection through lensless fiber-based planar antennas

Pietro Ernesto Lombardi, Ambra Giannetti, Paolo Cecchi, Francesco Chiavaioli, Steffen Howitz, Navid Soltani, Frank Sonntag, Costanza Toninelli, Mario Agio

Proceedings Volume 11772, 117720N (2021) https://doi.org/10.1117/12.2592502

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Proceedings Article | 5 March 2021 Presentation + Paper

PDMS-free modular plug and play construction kit for the development of micro-physiological systems

Stephan Behrens, Florian Schmieder, Christoph Polk, Patrick Schöps, Frank Sonntag

Proceedings Volume 11637, 116370O (2021) https://doi.org/10.1117/12.2585203

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Proceedings Article | 1 April 2020 Presentation

SEPSIS biomarker detection through fiber-based planar antennas (Conference Presentation)

Ambra Giannetti, Paolo Cecchi, Francesco Chiavaioli, Steffen Howitz, Pietro Lombardi, Navid Soltani, Frank Sonntag, Costanza Toninelli, Mario Agio

Proceedings Volume 11361, 113610Q (2020) https://doi.org/10.1117/12.2553234

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Proceedings Article | 2 March 2020 Presentation + Paper

Laser-induced cell injury in closed micro physiological systems: a novel method to study regeneration processes in vitro

Florian Schmieder, Deborah Förster, Mechthild Rösler, Jan Sradnick, Udo Klotzbach, Bernd Hohenstein, Frank Sonntag

Proceedings Volume 11268, 1126804 (2020) https://doi.org/10.1117/12.2566162

KEYWORDS: Injuries, In vitro testing, Systems modeling, Microfluidics, Laser systems engineering, Laser irradiation, Laser ablation, Lab on a chip

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Proceedings Article | 2 March 2020 Paper

Efficiency improvement of multilayer lab-on-a-chip production by dynamic beam shaping

Frank Sonntag, Thomas Kuntze, Patrick Schöps, Stephan Behrens, Florian Schmieder, Volker Franke, Udo Klotzbach

Proceedings Volume 11268, 112681C (2020) https://doi.org/10.1117/12.2542018

KEYWORDS: Mirrors, Scanners, Lab on a chip, Beam shaping, Control systems, Logic, Polymers, Interfaces, System on a chip, Laser processing

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Proceedings Article | 28 February 2017 Presentation + Paper

Microfluidic system for in-vitro hypoxia assays

M. Busek, S. Grünzner, T. Steege, C. Steinfelder, F. Schmieder, U. Klotzbach, F. Sonntag

Proceedings Volume 10061, 1006110 (2017) https://doi.org/10.1117/12.2253664

KEYWORDS: Hypoxia, Oxygen, Microfluidics, 3D modeling, Animal model studies, Systems modeling, In vitro testing, Lab on a chip, Mode conditioning cables, Animal testing, Nitrogen, Luminescence, Velocity measurements, Tissues, Gases

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Proceedings Article | 28 February 2017 Presentation + Paper

Lab-on-a-chip platform for high throughput drug discovery with DNA-encoded chemical libraries

S. Grünzner, F. Reddavide, C. Steinfelder, M. Cui, M. Busek, U. Klotzbach, Y. Zhang, F. Sonntag

Proceedings Volume 10061, 1006111 (2017) https://doi.org/10.1117/12.2253840

KEYWORDS: Industrial chemicals, Molecules, Signal processing, Chemistry, Biological research, Chemical analysis, Proteins, Solids, Thermodynamics, Signal to noise ratio

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The fast development of DNA-encoded chemical libraries (DECL) in the past 10 years has received great attention from pharmaceutical industries. It applies the selection approach for small molecular drug discovery. Because of the limited choices of DNA-compatible chemical reactions, most DNA-encoded chemical libraries have a narrow structural diversity and low synthetic yield. There is also a poor correlation between the ranking of compounds resulted from analyzing the sequencing data and the affinity measured through biochemical assays. By combining DECL with dynamical chemical library, the resulting DNA-encoded dynamic library (EDCCL) explores the thermodynamic equilibrium of reversible reactions as well as the advantages of DNA encoded compounds for manipulation/detection, thus leads to enhanced signal-to-noise ratio of the selection process and higher library quality. However, the library dynamics are caused by the weak interactions between the DNA strands, which also result in relatively low affinity of the bidentate interaction, as compared to a stable DNA duplex. To take advantage of both stably assembled dual-pharmacophore libraries and EDCCLs, we extended the concept of EDCCLs to heat-induced EDCCLs (hi-EDCCLs), in which the heat-induced recombination process of stable DNA duplexes and affinity capture are carried out separately. To replace the extremely laborious and repetitive manual process, a fully automated device will facilitate the use of DECL in drug discovery. Herein we describe a novel lab-on-a-chip platform for high throughput drug discovery with hi-EDCCL. A microfluidic system with integrated actuation was designed which is able to provide a continuous sample circulation by reducing the volume to a minimum. It consists of a cooled and a heated chamber for constant circulation. The system is capable to generate stable temperatures above 75 °C in the heated chamber to melt the double strands of the DNA and less than 15 °C in the cooled chamber, to reanneal the reshuffled library. In the binding chamber (the cooled chamber) specific retaining structures are integrated. These hold back beads functionalized with the target protein, while the chamber is continuously flushed with library molecules. Afterwards the whole system can be flushed with buffer to wash out unspecific bound molecules. Finally the protein-loaded beads with attached molecules can be eluted for further investigation.

Proceedings Article | 21 March 2016 Presentation + Paper

Universal lab-on-a-chip platform for complex, perfused 3D cell cultures

F. Sonntag, F. Schmieder, J. Ströbel, S. Grünzner, M. Busek, K. Günther, T. Steege, C. Polk, U. Klotzbach

Proceedings Volume 9705, 970516 (2016) https://doi.org/10.1117/12.2218606

KEYWORDS: Lab on a chip, Microfluidics, 3D printing, BioMEMS, Nonimpact printing, Tissue engineering, Rapid manufacturing, Medical diagnostics, Skin, Liver, Oxygen, Tissues, Manufacturing, Printing, Sensors, Laser applications, Embedded systems

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Proceedings Article | 1 March 2016 Paper

Label-free and fluorescence biosensing platform using one dimensional photonic crystal chips

F. Michelotti, S. Schmieder, A. Anopchenko, P. Munzert, A. Sinibaldi, R. Chandrawati, S. Rana, F. Sonntag, A. Occhicone, L. Napione, M. Stevens, E. Maillart, F.-E. Hibti, C. Frydman, N. Danz

Proceedings Volume 9750, 97501D (2016) https://doi.org/10.1117/12.2218517

KEYWORDS: Biosensing, Photonic crystals, Luminescence, Proteins, Surface plasmons, Waveguide sensors, Optical biosensors, Optical sensors, Biology, Electromagnetism, Ions, Charge-coupled devices, Signal detection, Sensors, Neodymium, Polymers, Fluorescent markers

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Proceedings Article | 5 May 2015 Paper

Biosensing platform combining label-free and labelled analysis using Bloch surface waves

Norbert Danz, Alberto Sinibaldi, Peter Munzert, Aleksei Anopchenko, Erik Förster, Stefan Schmieder, Rona Chandrawati, Riccardo Rizzo, Rene Heller, Frank Sonntag, Alessandro Mascioletti, Subinoy Rana, Thomas Schubert, Molly Stevens, Francesco Michelotti

Proceedings Volume 9506, 95060V (2015) https://doi.org/10.1117/12.2178444

KEYWORDS: Luminescence, Sensors, Thin films, Dielectrics, Silica, Proteins, Biosensing, Fluorescent markers, Charge-coupled devices, CCD image sensors

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Proceedings Article | 12 March 2015 Paper

Multilayer based lab-on-a-chip-systems for substance testing

Frank Sonntag, Stefan Grünzner, Florian Schmieder, Mathias Busek, Udo Klotzbach, Volker Franke

Proceedings Volume 9351, 93510C (2015) https://doi.org/10.1117/12.2083100

KEYWORDS: Microfluidics, Lab on a chip, Multilayers, Manufacturing, Laser bonding, Polymers, Laser applications, Laser development, Diffusion, Laser tissue interaction

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Proceedings Article | 26 May 2009 Paper

Novel lab-on-a-chip system for the label-free detection of DNA hybridization and protein-protein interaction by surface plasmon resonance (SPR)

F. Sonntag, S. Schmieder, N. Danz, M. Mertig, N. Schilling, U. Klotzbach, E. Beyer

Proceedings Volume 7365, 73650Q (2009) https://doi.org/10.1117/12.821467

KEYWORDS: Proteins, Gold, Microfluidics, Printing, Sensors, Surface plasmons, Lab on a chip, Molecules, Diagnostics, Integrated optics

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Proceedings Article | 26 May 2009 Paper

Characterization of novel micro fluidic approaches for increased sensitivity of sensors measuring surface reactions

F. Sonntag, M. Gruchow, F. Mehringer, S. Vogelsang, U. Klotzbach, E. Beyer

Proceedings Volume 7365, 73650P (2009) https://doi.org/10.1117/12.821463

KEYWORDS: Dielectrophoresis, Particles, Electrodes, Diffusion, Dielectrics, Sensors, Statistical analysis, Lab on a chip, Molecules, Systems modeling

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Proceedings Article | 20 March 2007 Paper

Requirements and potentialities of packaging for bioreactors with LTCC and polymers

Udo Klotzbach, Volker Franke, Frank Sonntag, Lothar Morgenthal, Eckhard Beyer

Proceedings Volume 6459, 645902 (2007) https://doi.org/10.1117/12.702206

KEYWORDS: Polymers, Ceramics, Sensors, Polymeric sensors, Pulsed laser operation, Nd:YAG lasers, Laser applications, Laser systems engineering, Lab on a chip, Packaging

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Proceedings Article | 15 July 2004 Paper

Influence of gas on cutting silicon with solid state laser

Udo Klotzbach, Stephanie Maelzer, Thomas Kuntze, Michael Panzner, Michael Doetschel, F. Sonntag, Eckhard Beyer

Proceedings Volume 5339, (2004) https://doi.org/10.1117/12.532133

KEYWORDS: Silicon, Laser cutting, Helium, Argon, Semiconductor lasers, Semiconducting wafers, Gases, Oxygen, Solid state lasers, Laser processing

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Showing 5 of 18 publications

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