KEYWORDS: 3D image processing, 3D displays, Digital holography, 3D image enhancement, Digital imaging, Speckle, Holography, Image enhancement, Scattering, Multiplexing, Digital micromirror devices, Light scattering
In digital 3D holographic displays, the generation of realistic 3D images has been hindered by limited viewing angle and image size. Here we demonstrate a digital 3D holographic display using volume speckle fields produced by scattering layers in which both the viewing angle and the image size are greatly enhanced. Although volume speckle fields exhibit random distributions, the transmitted speckle fields have a linear and deterministic relationship with the input field. By modulating the incident wavefront with a digital micro-mirror device, volume speckle patterns are controlled to generate 3D images of micrometer-size optical foci with 35° viewing angle in a volume of 2 cm × 2 cm × 2 cm.
Developing an efficient strategy for light focusing through scattering media is an important topic in the study of multiple light scattering. The enhancement factor of the light focusing, defined as the ratio between the optimized intensity and the background intensity is proportional to the number of controlling modes in a spatial light modulator (SLM). The demonstrated enhancement factors in previous studies are typically less than 1,000 due to several limiting factors, such as the slow refresh rate of a LCoS SLM, long optimization time, and lack of an efficient algorithm for high controlling modes. A digital micro-mirror device is an amplitude modulator, which is recently widely used for fast optimization through dynamic biological tissues. The fast frame rate of the DMD up to 16 kHz can also be exploited for increasing the number of controlling modes. However, the manipulation of large pattern data and efficient calculation of the optimized pattern remained as an issue.
In this work, we demonstrate the enhancement factor more than 100,000 in focusing through scattering media by using 1 Mega controlling modes of a DMD. Through careful synchronization between a DMD, a photo-detector and an additional computer for parallel optimization, we achieved the unprecedented enhancement factor with 75 mins of the optimization time. We discuss the design principles of the system and the possible applications of the enhanced light focusing.
Imaging brain tissues is an essential part of neuroscience because understanding brain structure provides relevant information about brain functions and alterations associated with diseases. Magnetic resonance imaging and positron emission tomography exemplify conventional brain imaging tools, but these techniques suffer from low spatial resolution around 100 μm. As a complementary method, histopathology has been utilized with the development of optical microscopy. The traditional method provides the structural information about biological tissues to cellular scales, but relies on labor-intensive staining procedures. With the advances of illumination sources, label-free imaging techniques based on nonlinear interactions, such as multiphoton excitations and Raman scattering, have been applied to molecule-specific histopathology. Nevertheless, these techniques provide limited qualitative information and require a pulsed laser, which is difficult to use for pathologists with no laser training.
Here, we present a label-free optical imaging of mouse brain tissues for addressing structural alteration in Alzheimer’s disease. To achieve the mesoscopic, unlabeled tissue images with high contrast and sub-micrometer lateral resolution, we employed holographic microscopy and an automated scanning platform. From the acquired hologram of the brain tissues, we could retrieve scattering coefficients and anisotropies according to the modified scattering-phase theorem. This label-free imaging technique enabled direct access to structural information throughout the tissues with a sub-micrometer lateral resolution and presented a unique means to investigate the structural changes in the optical properties of biological tissues.
We demonstrate that simultaneous application of optical clearing agents (OCAs) and complex wavefront shaping in optical coherence tomography (OCT) can provide significant enhancement of penetration depth and imaging quality. OCA reduces optical inhomogeneity of a highly scattering sample, and the wavefront shaping of illumination light controls multiple scattering, resulting in an enhancement of the penetration depth and signal-to-noise ratio. A tissue phantom study shows that concurrent applications of OCA and wavefront shaping successfully operate in OCT imaging. The penetration depth enhancement is further demonstrated for ex vivo mouse ears, revealing hidden structures inaccessible with conventional OCT imaging.
Multiple light scattering in tissue limits the penetration of optical coherence tomography (OCT) imaging. Here, we present in vivo OCT imaging of a live mouse using wavefront shaping (WS) to enhance the penetration depth. A digital micromirror device was used in a spectral-domain OCT system for complex WS of an incident beam which resulted in the optimal delivery of light energy into deep tissue. Ex vivo imaging of chicken breasts and mouse ear tissues showed enhancements in the strength of the image signals and the penetration depth, and in vivo imaging of the tail of a live mouse provided a multilayered structure inside the tissue.
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