Mr. Joshua T. Beckham Profile

Joshua T. Beckham

at Vanderbilt Univ

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Author

Publications (5)

SPIE Journal Paper | 1 September 2008 Open Access

In-vivo optical imaging of hsp70 expression to assess collateral tissue damage associated with infrared laser ablation of skin

Gerald Wilmink, Susan Opalenik, Joshua Beckham, Mark Mackanos, Lillian Nanney, Christopher Contag, Jeffrey Davidson, E. Duco Jansen

JBO, Vol. 13, Issue 05, 054066, (September 2008) https://doi.org/10.1117/12.10.1117/1.2992594

KEYWORDS: Tissues, Laser ablation, Free electron lasers, Skin, Laser tissue interaction, Optical imaging, Proteins, Green fluorescent protein, In vivo imaging, Luminescence

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Proceedings Article | 8 February 2007 Paper

Wavelength-dependent dynamics of heat shock protein 70 expression in free electron laser wounds

Gerald Wilmink, Joshua Beckham, Mark Mackanos, Christopher Contag, Jeffrey Davidson, E. Jansen

Proceedings Volume 6440, 644003 (2007) https://doi.org/10.1117/12.705085

KEYWORDS: Free electron lasers, Tissues, Laser tissue interaction, Absorption, Skin, Tissue optics, Proteins, Light scattering, Scattering, Collagen

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SPIE Journal Paper | 1 July 2006 Open Access

Assessing laser-tissue damage with bioluminescent imaging

Gerald Wilmink, Susan Opalenik, Josh Beckham, Jeffrey Davidson, Eric Jansen

JBO, Vol. 11, Issue 04, 041114, (July 2006) https://doi.org/10.1117/12.10.1117/1.2339012

KEYWORDS: Proteins, Gas lasers, Bioluminescence, Skin, Laser induced damage, Laser tissue interaction, Carbon monoxide, Tissues, Animal model studies, Microscopy

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Effective medical laser procedures are achieved by selecting laser parameters that minimize undesirable tissue damage. Traditionally, human subjects, animal models, and monolayer cell cultures have been used to study wound healing, tissue damage, and cellular effects of laser radiation. Each of these models has significant limitations, and consequently, a novel skin model is needed. To this end, a highly reproducible human skin model that enables noninvasive and longitudinal studies of gene expression was sought. In this study, we present an organotypic raft model (engineered skin) used in combination with bioluminescent imaging (BLI) techniques. The efficacy of the raft model was validated and characterized by investigating the role of heat shock protein 70 (hsp70) as a sensitive marker of thermal damage. The raft model consists of human cells incorporated into an extracellular matrix. The raft cultures were transfected with an adenovirus containing a murine hsp70 promoter driving transcription of luciferase. The model enables quantitative analysis of spatiotemporal expression of proteins using BLI. Thermal stress was induced on the raft cultures by means of a constant temperature water bath or with a carbon dioxide (CO2) laser (=10.6 µm, 0.679 to 2.262 W/cm2, cw, unfocused Gaussian beam, L=4.5 mm, 1 min exposure). The bioluminescence was monitored noninvasively with an IVIS 100 Bioluminescent Imaging System. BLI indicated that peak hsp70 expression occurs 4 to 12 h after exposure to thermal stress. A minimum irradiance of 0.679 W/cm2 activated the hsp70 response, and a higher irradiance of 2.262 W/cm2 was associated with a severe reduction in hsp70 response due to tissue ablation. Reverse transcription polymerase chain reaction demonstrated that hsp70 mRNA levels increased with prolonged heating exposures. Enzyme-linked immunosorbent protein assays confirmed that luciferase was an accurate surrogate for hsp70 intracellular protein levels. Hematoxylin and eosin stains verified the presence of the ...

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Proceedings Article | 27 August 2003 Paper

Bioluminescence imaging as a marker for cellular Hsp70 response to thermal laser injury

Joshua Beckham, Jennifer Baran, Mark Mackanos, Cornelia Crooke, Takamune Takahashi, Caitlin O'Connell-Rodwell, Christopher Contag, E. Duco Jansen

Proceedings Volume 4961, (2003) https://doi.org/10.1117/12.502190

KEYWORDS: Proteins, Photon counting, Bioluminescence, Tissues, Cell death, Laser irradiation, Injuries, Flow cytometry, Time correlated photon counting, Pulsed laser operation

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Proceedings Article | 9 July 2001 Paper

Optical imaging of Hsp70 gene expression following thermal laser injury

Josh Beckham, J. Baran, A. Izzo, E. Duco Jansen

Proceedings Volume 4257, (2001) https://doi.org/10.1117/12.434723

KEYWORDS: Proteins, Laser irradiation, Pulsed laser operation, Injuries, Temperature metrology, Laser tissue interaction, Photon counting, Cameras, Absorption, Bioluminescence

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