Metabolic features of mitosis remain poorly understood because this phase of the cell cycle is rapid and heterogeneous between cells within a dish. Label-free optical metabolic imaging (OMI) can monitor rapid changes in cell metabolism with single cell resolution using two-photon microscopy of the optical redox ratio (NAD(P)H/FAD) and NAD(P)H fluorescence lifetimes. Here, we brought together image analysis tools to quantify OMI time-courses of single cells undergoing mitosis across multiple cell lines. Alignment of OMI and morphological features over time provided unique insight into metabolic changes during mitosis within unperturbed systems.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.