The determination of selenocysteine was investigated based on the fact that the -SeH bond in selenols can specifically undergo nucleophilic addition reaction with the Se-N bond in benzoselenadiazole. The results obtained showed that the pH of t determination system was 4.7 and the optimum temperature was 37°C. The linear relationship was F=90.246c+298.36, and the correlation coefficient R2 was 0.9916. The relative error of this method was less than 2.2%, and the recoveries were in the range of 96.6%~103%. This experimental method is based on the nucleophilic addition reaction of -SeH, which enables the rapid and accurate determination of selenocysteine without catalysis, heating and long waiting time. This approach provides some reference for the study of the physiological significance of selenium.
As an important restoration substance in cells, reduced glutathione (GSH) plays an important physiological role in the human body. In this paper, GSH was determined by indirect Spectrophotometry. Through the optimization experimental conditions, the amount of Fe3+-1,10-phenanthroline chromogenic concentration was 0.8 mg/mL, and the optimum pH value was 4.0 mL, and the amount of buffer solution was 5.0 mL, and the heating time and cooling time were 10 min. In addition, the absorbance was measured at 510 nm, and GSH was in the range of 0~0.4 mg/mL. The linear regression equation was A=1.7981c+0.1348 (which A represents the absorbance and c represents the concentration of GSH), and the correlation coefficient was r2 = 0.9955. The method was applied to the determination of reduced GSH in three GSH samples. The indirect spectrophotometry method is simple, low cost and easy to operate.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.