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In this study, we utilized multiphoton microscopy to image three distinct tumor-associated collagen signatures (TACS) at the invasive front of the tumor. We then used MATLAB to extract the corresponding collagen morphological features and analyzed their correlation with clinical staging. Our results revealed significant changes in the morphological features of collagen fibers in TACS across different stages of BC at the tumor invasion front. Notably, the proportionate area and number of collagen fibers were found to be inversely correlated with the clinical staging risk group of the disease. Our findings offer new perspectives for the clinical staging of BC, providing valuable insights that may enhance the predictive accuracy of disease progression and prognostic outcomes.
Effects of cholesterol depletion on membrane nanostructure in MCF-7 cells by atomic force microscopy
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