Ablation rate of polymers in laser ablation-based microfabrication depends on both laser parameters and polymer characteristics. This study aims to establish a scaling relationship linking the ablation rate and the properties of polymers commonly used in microfluidics. Ablation rate of polymers was determined experimentally using 193nm, 248nm and 308nm radiations. Polymer descriptors included thermal and surface properties. Statistical analysis was carried out for laser fluence against various polymer descriptors and/or their combinations. Analysis results show a relatively high correlation coefficient of 0.82 for the polymer ablation data when we compare fluence against the product of ablation rate and the difference between glass transition temperature and room temperature.
Microarray-like chips are based on effective immobilisation of surface biomolecules and preservation of their bioactivities. We have applied the concept of Connolly molecular surface for modelling and computation of the pure surface properties of proteins, which are of fundamental importance to surface-based protein science and engineering, especially for protein microarray chips. This is achieved by de-convoluting various molecular interaction components into single surface elements, and integrating a specific property of the atoms mostly close to the surface element to obtain the pure surface property of a protein. A methodology for obtaining electron charge, hydrophobicity as well as a-helix and b-pleated sheet structural indices has been developed. A parallel study shows that this technique is useful for modelling and computation of protein-protein and protein-polymer interactions, including protein attachment in molecularly confined spaces.
An attempt to simulate the interaction between an AFM tip and a protein surface by employing the concept of Connolly molecular surface with a carbon probe has been investigated. A methodology has been developed to permit the computation of the Connolly surface for a protein, where numerous atoms are simultaneously interacting each other. The van der Waals and electrostatic interactions between the probe and the relevant Connolly surface elements are integrated to obtain the total interaction, resulting in a precise theoretical account for a variety of interaction components. The simulation offers a meaningful opportunity for AFM scientists to interpret AFM surface mapping results more precisely or on a more general level the polymer surface-protein surface interactions.
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