Low concentration Phloxine B staining was applied for nonlinear microscope mosaic imaging of skin alterations in Pseudoxanthoma Elasticum sections helping in determination of illness severity by quantitative analysis of calcium deposits in the skin.
A fiber coupled, sub ps Ti:sapphire laser suitable for in vivo, stain free, 3D imaging of skin alterations is introduced. It is pumped by a low cost, 2.1 W pump laser and delivers 0.6 1 ps high peak power pulses optimized for fiber delivery.
FFT analysis on mosaic SHG images of basal cell carcinoma skin sections of different subtypes is presented. This analysis combined with two-photon auto-fluorescence imaging might be useful for assessment of tumor borders.
Skin cancer is the most common type of malignant tumors in humans. Early diagnosis is the key to successful surgical treatment. In this work we present a non-invasive screening tool for early stage detection of skin cancer and also for the evaluation of post-operative scars.
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