We introduce a compact attachment for microscope objectives that allows for the conversion of conventional fluorescence microscopes into Airy light-sheet microscopes. The attachment includes a one-dimensional Airy beam generator, which comprises a gradient-index collimator and a 3D nano-printed cubic phase-plate, realized through two-photon polymerization 3D nano-printing and a two-step writing process that guarantees an optical-quality surface for the phase plate. The micro-optical unit is affixed to a mechanical holder equipped with micro-stages, thereby facilitating the unit's integration into commercial microscopes. The implementation and imaging performance of this system and its fundamental imaging characteristics are discussed, with findings based on diverse samples.
Beam-shaping through optical fibers facilitates miniaturized light delivery in fields such as endoscopy, communication, etc. As the stability of optical beam delivery and collection depends a lot on the fiber stability, the impact of fiber deformations on mode-coupling for different types of fibers should be analyzed. To this end, we present a numerical simulation tool modeling optical field propagation through fibers with arbitrary refractive index profiles, focusing on fiber twisting, tapering, and bending. Our simulation tool is compared against the state-of-the-art simulation software evaluating computational efficiency, versatility, and user-friendliness. The simulation tool is gratis, open-source, fast, and supports optional CUDA acceleration.
Attenuation of optical fields owing to scattering and absorption limits the penetration depth into tissue. Whilst aberration correction may be used this is difficult to implement over a large field of view in heterogeneous tissue. Recently, the novel approach of attenuation-compensation of propagation-invariant light fields has shown increase in depth penetration for light-sheet microscopy. Here we show this powerful approach may be implemented in a facile manner utilizing a graded neutral density filter circumventing the need for expensive beam shaping apparatus. A ‘gold standard’ system utilizing a spatial light modulator for beam shaping is used to benchmark our low-cost implementation.
Miniaturisation of endoscopes can be achieved using lensless endoscope probes, which enhances in vivo deep- tissue imaging technology. The necessity of a detailed understanding of light propagation through optical fibres is paramount, since beam focusing and scanning at tissue require beam shaping at the proximal end of the fibre. For stable light delivery and collection, the sensitivity of various fibre profiles against fibre deformations needs to be reviewed. We present a numerical simulation tool investigating optical field propagation through multimode and multicore optical fibres, emphasizing fibre-bending deformations. The simulation tool enables user to choose optimum fibre with best possible realistic parameters for any application.
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