Acoustofluidics exploits ultrasounds and microfluidic platforms to achieve label-free and contactless manipulation of micro sized objects. Here, we demonstrate the use of off-axis digital holography to investigate the behavior of erythrocytes dispersed in water and exposed to ultrasound standing waves. We consider two different regimes of manipulation. In the first case, the sample is stilled inside the microfluidic channel. Under the influence of acoustic forces, the cells move to the first nodal plane, where they start an aggregation process. We follow the formation of clusters in different regions of the channel, highlighting the different structures that emerge. As a second regime, we monitor the axial position of cells flowing during the application of ultrasuonds. By using a resonance frequency that originates multiple nodal positions, we show how holographic imaging can be used to image the cells distributed in the different nodes.
Acoustophoresis devices are popular tools for manipulation and diagnostic in microfluidic environments. They offer the opportunity for contactless manipulation of cells. We demonstrate that the combination of acoustic manipulation and holographic imaging provides a suitable system for the simultaneous handling and of biological matter. We employ an acoustofluidic device with a transparent piezo element, to enable optical investigation through the channel. The holographic imaging is thus employed to observe and analyze the behavior of Red Blood Cells during the application of ultrasound radiation. The flexible refocusing, and quantitative phase imaging of single cells and RBCs clusters is reported.
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