Dr. Mark A. Mackanos Profile

Dr. Mark A. Mackanos

VP of Engineering

SPIE Involvement:

Author

Publications (19)

SPIE Journal Paper | 1 July 2011 Open Access

Pulse duration determines levels of Hsp70 induction in tissues following laser irradiation

Mark Mackanos, Christopher Contag

JBO, Vol. 16, Issue 07, 078002, (July 2011) https://doi.org/10.1117/12.10.1117/1.3600013

KEYWORDS: Pulsed laser operation, Tissues, Gas lasers, In vivo imaging, Skin, Cameras, Temperature metrology, Proteins, Carbon monoxide, Injuries

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SPIE Journal Paper | 1 May 2011 Open Access

Image-guided genomic analysis of tissue response to laser-induced thermal stress

Mark Mackanos, Mike Helms, Flora Kalish, Christopher Contag

JBO, Vol. 16, Issue 05, 058001, (May 2011) https://doi.org/10.1117/12.10.1117/1.3573387

KEYWORDS: Proteins, Laser tissue interaction, Tissues, Control systems, Biological research, Receptors, Image analysis, Injuries, Cell death, Inflammation

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SPIE Journal Paper | 1 July 2009 Open Access

Laser-induced disruption of systemically administered liposomes for targeted drug delivery

Mark Mackanos, Malika Larabi, Rajesh Shinde, Dmitrii Simanovskii, Samira Guccione, Christopher Contag

JBO, Vol. 14, Issue 04, 044009, (July 2009) https://doi.org/10.1117/12.10.1117/1.3174410

KEYWORDS: Bioluminescence, In vivo imaging, Imaging systems, Tissues, Neodymium lasers, Laser tissue interaction, Molecules, Temperature metrology, Luminescence, Forward looking infrared

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SPIE Journal Paper | 1 July 2009 Open Access

Use of an endoscope-compatible probe to detect colonic dysplasia with Fourier transform infrared spectroscopy

Mark Mackanos, John Hargrove, Rolf Wolters, Christine Du, Shai Friedland, Roy Soetikno, Christopher Contag, May Arroyo, James Crawford, Thomas Wang

JBO, Vol. 14, Issue 04, 044006, (July 2009) https://doi.org/10.1117/12.10.1117/1.3174387

KEYWORDS: FT-IR spectroscopy, Optical fibers, Tissues, Proteins, Silver, Absorbance, Endoscopy, Algorithm development, Binary data, Spectroscopy

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Proceedings Article | 3 March 2009 Paper

Fiber optic FTIR instrument for in vivo detection of colonic neoplasia

Matthew Van Nortwick, John Hargrove, Rolf Wolters, James Crawford, May Arroyo, Mark Mackanos, Christopher Contag, Thomas Wang

Proceedings Volume 7172, 71720K (2009) https://doi.org/10.1117/12.808570

KEYWORDS: FT-IR spectroscopy, Tissues, Optical fibers, Endoscopy, Silver, Biopsy, Absorbance, Cancer, Absorption, Endoscopes

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SPIE Journal Paper | 1 September 2008 Open Access

In-vivo optical imaging of hsp70 expression to assess collateral tissue damage associated with infrared laser ablation of skin

Gerald Wilmink, Susan Opalenik, Joshua Beckham, Mark Mackanos, Lillian Nanney, Christopher Contag, Jeffrey Davidson, E. Duco Jansen

JBO, Vol. 13, Issue 05, 054066, (September 2008) https://doi.org/10.1117/12.10.1117/1.2992594

KEYWORDS: Tissues, Laser ablation, Free electron lasers, Skin, Laser tissue interaction, Optical imaging, Proteins, Green fluorescent protein, In vivo imaging, Luminescence

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SPIE Journal Paper | 1 May 2008 Open Access

In vivo analysis of heat-shock-protein-70 induction following pulsed laser irradiation in a transgenic reporter mouse

Caitlin O'Connell-Rodwell, Mark Mackanos, Dmitrii Simanovski, Yu-An Cao, Michael Bachmann, H. Alan Schwettman, Christopher Contag

JBO, Vol. 13, Issue 03, 030501, (May 2008) https://doi.org/10.1117/12.10.1117/1.2904665

KEYWORDS: Tissues, In vivo imaging, Proteins, Gas lasers, Pulsed laser operation, Injuries, Skin, Bioluminescence, Carbon monoxide, Laser applications

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Proceedings Article | 8 February 2007 Paper

Wavelength-dependent dynamics of heat shock protein 70 expression in free electron laser wounds

Gerald Wilmink, Joshua Beckham, Mark Mackanos, Christopher Contag, Jeffrey Davidson, E. Jansen

Proceedings Volume 6440, 644003 (2007) https://doi.org/10.1117/12.705085

KEYWORDS: Free electron lasers, Tissues, Laser tissue interaction, Absorption, Skin, Tissue optics, Proteins, Light scattering, Scattering, Collagen

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SPIE Journal Paper | 1 July 2006 Open Access

Effect of optical tissue clearing on spatial resolution and sensitivity of bioluminescence imaging

Eric Jansen, Patrick Pickett, Mark Mackanos, Jack Virostko

JBO, Vol. 11, Issue 04, 041119, (July 2006) https://doi.org/10.1117/12.10.1117/1.2337651

KEYWORDS: Skin, In vivo imaging, Spatial resolution, Bioluminescence, Scattering, Tissue optics, Light scattering, In vitro testing, Cameras, Tissue clearing

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Proceedings Article | 18 April 2005 Paper

Corneal ablation using the pulse stretched free electron laser

Mark Mackanos, Karen Joos, John Kozub, E. Jansen

Proceedings Volume 5688, (2005) https://doi.org/10.1117/12.596603

KEYWORDS: Picosecond phenomena, Free electron lasers, Laser ablation, Cornea, Tissues, Optical fibers, Statistical analysis, Eye, Laser sources, Absorption

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The Mark-III Free Electron Laser (FEL), tuned to λ=6.45 μm has been demonstrated to provide for efficient ablation in ocular and neural tissues with minimal collateral damage. To date, the role of the FEL pulse structure on the mechanism of ablation has not been determined. In an effort to study the role of the FEL micropulse on the ablation of corneal tissue, the native pulse structure of the FEL, a 2.85 gigahertz repetition of picosecond pulses within a five microsecond macropulse envelope, was changed using a a pulse stretcher. This device changes the duration of the micropulse from 1 picosecond to 30-200 picoseconds in length, thus reducing the peak intensity of the micropulse by as much as 200x the original intensity, while the macropulse energy remains unchanged. Two basic metrics were studied: the ablation threshold on water and the ablation crater depth on gelatin. These metrics were employed at λ=6.45 and 6.1 μm for 1, 100, and 200 picoseconds in micropulse duration. The results showed a very slight difference between the 1, 100, and 200 picosecond micropulse duration, given a 200 fold decrease in peak energy for both the threshold and crater depth measurements. Brightfield imaging was also performed to probe the ablation dynamics and showed no difference between the 1 and 200 ps micropulses. The effect of changing the micropulse duration was studied on the ablation of canine cornea. Craters (500 micron diameter) were created with 25 pulses at three times the ablation threshold as determined for water on freshly enucleated corneas within 12 hours of removal. Three rows of seven craters were created on the center of each cornea. The native one picosecond micropulse and 200 picosecond stretched micropulse were compared at λ=6.1 and 6.45 μm. Histological data shows that less thermal damage is present at 6.1 μm compared with 6.45 μm; however, there is no significant difference between the native and stretched pulses with respect to thermal damage.

Proceedings Article | 15 April 2005 Paper

Analysis of soft tissue ablation using the pulse stretched free electron laser

Mark Mackanos, John Kozub, Darrel Ellis, E. Duco Jansen

Proceedings Volume 5695, (2005) https://doi.org/10.1117/12.596608

KEYWORDS: Picosecond phenomena, Free electron lasers, Laser ablation, Laser tissue interaction, Tissues, Skin, Pulsed laser operation, Laser sources, Statistical analysis, Ultraviolet radiation

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Proceedings Article | 15 April 2005 Paper

Comparison of OPO and Mark-III FEL for mid-infrared soft tissue ablation

Mark Mackanos, Dmitrii Simanovski, H. Alan Schwettman, E. Duco Jansen

Proceedings Volume 5695, (2005) https://doi.org/10.1117/12.597215

KEYWORDS: Free electron lasers, Laser ablation, Optical parametric oscillators, Pulsed laser operation, Laser sources, Tissues, Mid-IR, Laser damage threshold, Laser tissue interaction, Picosecond phenomena

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Proceedings Article | 1 July 2004 Paper

Effect of FEL micropulse duration on ablation characteristics at 6.45 um

Mark Mackanos, John Kozub, E. Jansen

Proceedings Volume 5319, (2004) https://doi.org/10.1117/12.535803

KEYWORDS: Picosecond phenomena, Free electron lasers, Laser ablation, Tissues, Skin, Laser tissue interaction, Optical coherence tomography, Statistical analysis, Pulsed laser operation, Error analysis

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Proceedings Article | 1 July 2004 Paper

Ablation of soft tissue at 6.45 um using a strontium vapor laser

Mark Mackanos, Borislav Ivanov, Anatoly Soldatov, I. Kostadinov, Marcus Mendenhall, David Piston, Richard Haglund, E. Jansen

Proceedings Volume 5319, (2004) https://doi.org/10.1117/12.535479

KEYWORDS: Laser ablation, Laser tissue interaction, Strontium, Free electron lasers, Strontium vapor lasers, Pulsed laser operation, Mirrors, Superposition, Laser development, Tissues

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Proceedings Article | 1 June 2004 Paper

Effect of micropulse duration on tissue ablation using a stretched free electron laser pulse train

John Kozub, Mark Mackanos, Marcus Mendenhall, E. Duco Jansen

Proceedings Volume 5340, (2004) https://doi.org/10.1117/12.535893

KEYWORDS: Free electron lasers, Laser ablation, Picosecond phenomena, Tissues, Laser tissue interaction, Absorption, Pulsed laser operation, Spectroscopy, Skin, Optical coherence tomography

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SPIE Journal Paper | 1 October 2003 Open Access

Delivery of midinfrared (6 to 7-μm) laser radiation in a liquid environment using infrared-transmitting optical fibers

Mark Mackanos, E. Duco Jansen, Brandon Shaw, Jasbinder Sanghera, Ishwar Aggarwal, Abraham Katzir

JBO, Vol. 8, Issue 04, (October 2003) https://doi.org/10.1117/12.10.1117/1.1606460

KEYWORDS: Tissues, Liquids, Silver, Absorption, Laser ablation, Free electron lasers, Arsenic, Fiber lasers, Laser damage threshold, Laser tissue interaction

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Proceedings Article | 27 August 2003 Paper

Bioluminescence imaging as a marker for cellular Hsp70 response to thermal laser injury

Joshua Beckham, Jennifer Baran, Mark Mackanos, Cornelia Crooke, Takamune Takahashi, Caitlin O'Connell-Rodwell, Christopher Contag, E. Duco Jansen

Proceedings Volume 4961, (2003) https://doi.org/10.1117/12.502190

KEYWORDS: Proteins, Photon counting, Bioluminescence, Tissues, Cell death, Laser irradiation, Injuries, Flow cytometry, Time correlated photon counting, Pulsed laser operation

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Proceedings Article | 14 July 2003 Paper

Fiber-delivered mid-infrared (6-7 µm) laser ablation of retinal tissue under perfluorodecalin

Mark Mackanos, Karen Joos, E. Duco Jansen

Proceedings Volume 4951, (2003) https://doi.org/10.1117/12.482369

KEYWORDS: Laser ablation, Tissues, Retina, Optical fibers, Silver, Absorption, Free electron lasers, Vitreous, Eye, Laser tissue interaction

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Proceedings Article | 9 July 2001 Paper

Effects of IR wavelength on ablation mechanics: a study of acoustic signals

Stephen Uhlhorn, David Mongin, Mark Mackanos, E. Duco Jansen

Proceedings Volume 4257, (2001) https://doi.org/10.1117/12.434702

KEYWORDS: Acoustics, Laser ablation, Free electron lasers, Absorption, Proteins, Laser tissue interaction, Tissues, Infrared lasers, Laser energy, Mechanics

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Showing 5 of 19 publications

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