A multilayered model of the optical anisotropy of the light-scattering layer of biological tissue is considered. The Muller matrix of the depolarizing layer is represented as a superposition of partial matrix operators for linear and circular birefringence-dichroism. For multiple scattering, an algorithm is proposed for the expansion of the Muller matrix in the form of two components. The first is the fully polarized component of the Muller matrix. The second is the completely depolarized component of the Muller matrix. The algorithms for measuring the elements of the fully polarized component of the Muller matrix for distributions of the phase and amplitude anisotropy of the depolarizing biological tissue are found. Maps of the distributions of the completely polarized component of the Muller matrix elements of histological sections of healthy and diabetic rats liver tissue have been studied. Sensitivity, specificity and balanced accuracy of the Muller-matrix reconstruction method of the polycrystalline structure of multiply scattering biological tissues are determined. Within the framework of the statistical analysis of the maps of the elements of the fully polarized component of the Muller matrix, histological sections of the liver tissue, objective criteria for the differentiation of healthy and diabetic rats have been found.
An optical-physical system for extracting information about the fluctuations in the optical anisotropy of strongly scattering biological tissues is considered. A model is proposed for the formation of a depolarized background by birefringent and dichroic structures. An explicit form and symmetry of the completely depolarized component of the Mueller matrix is determined, a second-order differential matrix. An algorithm for the analytic determination of the distributions of elements of a second-order differential matrix is found. Interrelations between the magnitude of the elements of the second-order differential matrix and the fluctuations of the linear and circular birefringence-dichroism are obtained. The technique of diffuse tomography of an optically anisotropic component of strongly scattering biological tissues has been developed and experimentally tested. Maps of the distributions of the elements of the completely depolarized component of the Mueller matrix of the histological sections of the internal organs of the healthy and of the diabetic rats . The sensitivity, specificity and balanced accuracy of the method of diffuse tomography of the polycrystalline structure of tumors of the uterine wall and the degree of hemorrhage of the liver are determined.
A model of multilayer polycrystalline structure of films of biological fluids of human organs has been developed. Each layer is associated with a partial Jones-matrix operator of phase and amplitude anisotropy. A new principle for detecting polarization-inhomogeneous object fields using a coherent laser wave is proposed. Algorithms for digital holographic reconstruction of field distributions of complex amplitudes in the plane of a polycrystalline film of a biological fluid are found. A new optical technique is proposed: direct measurement of 3D distributions of elements of the Jones matrix. Maps of layer-by-layer distributions of elements of the Jones matrix of polycrystalline urine films are studied. Sensitivity, specificity and balanced accuracy of the 3D Jones-matrix tomography method of the polycrystalline structure of urine films of healthy donors and patients with albuminuria were determined. Within the framework of the statistical analysis of stratified maps of elements of the Jones matrix of polycrystalline urine films, objective criteria for the early diagnosis of the onset and course of albuminuria were found.
The paper consists of two parts. The first part is devoted to the short theoretical basics of the method of differential Mueller-matrix description of properties of partially depolarizing layers. It was provided the experimentally measured maps of differential matrix of the 1st order of polycrystalline structure of the histological section of brain tissue. It was defined the statistical moments of the 1st-4th orders, which characterize the distribution of matrix elements. In the second part of the paper it was provided the data of statistic analysis of birefringence and dichroism of the histological sections of mice liver tissue (normal and with diabetes). It were defined the objective criteria of differential diagnostics of diabetes.
The paper consists of two parts. The first part presents short theoretical basics of the method of Jones-matrix mapping with the help of reference wave. It was provided experimentally measured coordinate distributions of modulus of Jones-matrix elements of polycrystalline film of bile. It was defined the values and ranges of changing of statistic moments, which characterize such distributions. The second part presents the data of statistic analysis of the distributions of matrix elements of polycrystalline film of urine of donors and patients with albuminuria. It was defined the objective criteria of differentiation of albuminuria.
The paper presents the results of Jones-matrix mapping of uterine wall histological sections with second-degree and third-degree endometriosis. The technique of experimental measurement of coordinate distributions of the modulus and phase values of Jones matrix elements is suggested. Within the statistical and cross-correlation approaches the modulus and phase maps of Jones matrix images of optically thin biological layers of polycrystalline films of plasma and cerebrospinal fluid are analyzed. A set of objective parameters (statistical and generalized correlation moments), which are the most sensitive to changes in the phase of anisotropy, associated with the features of polycrystalline structure of uterine wall histological sections with second-degree and third-degree endometriosis are determined.
The theoretical background of azimuthally stable method of Jones-matrix mapping of histological sections of biopsy of myocardium tissue on the basis of spatial frequency selection of the mechanisms of linear and circular birefringence is presented. The diagnostic application of a new correlation parameter – complex degree of mutual anisotropy – is analytically substantiated. The method of measuring coordinate distributions of complex degree of mutual anisotropy with further spatial filtration of their high- and low-frequency components is developed. The interconnections of such distributions with parameters of linear and circular birefringence of myocardium tissue histological sections are found. The comparative results of measuring the coordinate distributions of complex degree of mutual anisotropy formed by fibrillar networks of myosin fibrils of myocardium tissue of different necrotic states – dead due to coronary heart disease and acute coronary insufficiency are shown. The values and ranges of change of the statistical (moments of the 1st – 4th order) parameters of complex degree of mutual anisotropy coordinate distributions are studied. The objective criteria of differentiation of cause of death are determined.
The results of optical modeling of biological tissues polycrystalline multilayer networks have been presented. Algorithms of reconstruction of parameter distributions were determined that describe the linear and circular birefringence. For the separation of the manifestations of these mechanisms we propose a method of space-frequency filtering. Criteria for differentiation of benign and malignant tissues of the women reproductive sphere were found.
The work consists of investigation results of diagnostic efficiency of a new azimuthally stable Muellermatrix method of analysis of laser autofluorescence coordinate distributions of biological tissues histological sections. A new model of generalized optical anisotropy of biological tissues protein networks is proposed in order to define the processes of laser autofluorescence. The influence of complex mechanisms of both phase anisotropy (linear birefringence and optical activity) and linear (circular) dichroism is taken into account. The interconnections between the azimuthally stable Mueller-matrix elements characterizing laser autofluorescence and different mechanisms of optical anisotropy are determined. The statistic analysis of coordinate distributions of such Mueller-matrix rotation invariants is proposed. Thereupon the quantitative criteria (statistic moments of the 1st to the 4th order) of differentiation of histological sections of myometrium – group 1 (fibromyoma) and group 2 (adenocarcinoma) are estimated.
The model of Mueller-matrix description of mechanisms of optical anisotropy typical for polycrystalline films of liquor - optical activity, birefringence, as well as linear and circular dichroism - is suggested. Within the statistical analysis of such distributions the objective criteria of differentiation of films of liquor from the dead you people different times were determined. From the point of view of probative medicine the operational characteristics (sensitivity, specificity and accuracy) of the method of Mueller-matrix reconstruction of optical anisotropy parameters were found and its efficiency in another task - diagnostics of diseases of internal organs of rats was demonstrated.
A new information optical technique of diagnostics of the structure of polycrystalline films of blood plasma is
proposed. The model of Mueller-matrix description of mechanisms of optical anisotropy of such objects as optical
activity, birefringence, as well as linear and circular dichroism is suggested. The ensemble of informationally topical
azimuthally stable Mueller-matrix invariants is determined. Within the statistical analysis of such parameters
distributions the objective criteria of differentiation of films of blood plasma taken from healthy women and breast
cancer patients were determined. From the point of view of probative medicine the operational characteristics
(sensitivity, specificity and accuracy) of the information-optical method of Mueller-matrix mapping of polycrystalline
films of blood plasma were found and its efficiency in diagnostics of breast cancer was demonstrated. Considered the
prospects of applying the method in experimental medicine for differentiation of tissues of internal organs of healthy and
diabetic rats.
The principles of optical model of human bile polycrystalline structure are described. The results of investigating the interrelation between the values of statistical, correlation and fractal parameters are presented. They characterize the coordinate distributions of mutual polarization degree of the points of laser images of bile smears of cholelithiasis patients in combination with other pathologies. The diagnostic criteria of the cholelithiasis nascency and its severity degree differentiation are determined.
This research is aimed to investigate the reliability of Mueller-matrix differentiation of birefringence
change of optically thick layers of biological tissues at the early stages of the change in their physiological
state. This is performed by measuring the set of asymmetry and excess values of Mueller matrix image of the
phase element M44 in various points of the object under investigation.
The model of laser polarization fluorescence of biological tissues considering the mechanisms of
optically anisotropic absorption – linear and circular dichroism of protein networks was suggested.Muellermatrix
rotation invariants characterizing polarization manifestations of laser fluorescence are determined.The
interconnections between the statistical, correlation and fractal parameters characterizing the Mueller-matrix
images of laser polarization fluorescence and the peculiarities of the mechanisms of optically anisotropic
absorption of histological sections of uterus wall biopsy were found. Effectiveness of the method of azimuthinvariant
Mueller-matrix mapping of laser polarization fluorescence of protein networks in the task of
differentiation of benign and malignant tumors of uterus wall was demonstrated.
Our work is aimed at searching the possibilities to perform diagnostics and differentiation of structures inherent to liquid-crystal networks of blood plasma with various pathologies (health - breast cancer) by using the method to determine the coordinate distributions of phase shifts (phase maps) between orthogonal components of laser radiation amplitudes with the following statistical, fractal and singular analyses of these distributions.
The optical model of polycrystalline networks of blood plasma proteins is suggested. The results of investigating the
interrelation between the values of correlation (correlation area, asymmetry coefficient and autocorrelation function
excess) and fractal (dispersion of logarithmic dependencies of power spectra) parameters are presented. They
characterize the coordinate distributions of polarization azimuth of laser images of blood plasma smears and pathological
state of the organism. The diagnostic criteria of breast cancer nascency are determined.
This work presents the results of investigation of the efficiency of the method of both concerted spatial-frequency and
polarization-phase filtration of blood plasma proteins birefringence during the diagnostics of cancer of intestinum wall.
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