The paper presents the results of experimental testing of methods for azimuthal-invariant polarization mapping of laser induced microscopic images of fluorophores in histological sections of the liver of deceased; time monitoring of changes in the magnitude of statistical moments of the 1st - 4th orders characterizing the distributions of the azimuth and ellipticity of polarization of microscopic images of histological sections of the liver with different age of damage; determination of the diagnostic efficiency (time interval and accuracy) of establishing the age of damage to human internal organs by digital histological methods of mapping maps of azimuth and ellipticity of polarization of microscopic images of samples of histological sections of the brain, liver and kidney, as well as myocardium and lung tissue.
The results of applying algorithms for reconstructing parameters characterizing the linear dichroism of networks of biological crystals in differentiating changes in optical anisotropy associated with varying degrees of severity of pathology, pre-cancerous (atrophy and endometrial polyp) states of the cervix are presented.
This section contains the results of a study of the relationships between the 3D distributions of the optical anisotropy parameters of polycrystalline networks of biological fluid films of different biochemical composition and the layered phase sections of volume distributions of the magnitude and parameter phase of the "two-point" Stokes vector of the microscopic image.
A theoretical basis for the method of polarization-interference mapping of optically thin polycrystalline films of human biological fluids is given. The coordinate distributions of the value of the local contrast of the interference distributions of the polarization-inhomogeneous microscopic images of polycrystalline films of the synovial fluid of the human joint are investigated. In the framework of the statistical (statistical moments of the 1st-4th order) approaches, objective criteria for the distribution of the values of local contrast are established. The possibility of differentiation of weak changes in the optical anisotropy of blood films of healthy and patients with breast cancer patients is determined.
A theoretical basis for the method of polarization-interference mapping of optically thin polycrystalline films of human biological fluids is given. The coordinate distributions of the value of the local contrast of the interference distributions of the polarization-inhomogeneous microscopic images of polycrystalline films of the synovial fluid of the human joint are investigated. In the framework of the statistical (statistical moments of the 1st-4th order) approaches, objective criteria for the distribution of the values of local contrast are established. The possibility of differentiation of weak changes in the optical anisotropy of blood films of healthy and patients with breast cancer patients is determined.
The materials of experimental studies of the coordinate and statistical structure of the coordinate distributions of the degree of local depolarization of coprofiltrate layers taken from patients of group 1 and group 2 are presented.
The interrelations between the values of the statistical moments characterizing the coordinate distributions of the degree of local depolarization of coprofiltrate layers and the physiological state of the patients within group 1 and group 2 are established.
An analysis of the operational characteristics of the power of mapping the coordinate distributions of the degree of local depolarization of coprofiltrate layers taken from patients of group 1 and group 2 based on the determination of sensitivity, specificity, accuracy, predictability of positive and predictability of a negative result is carried out from the standpoint of evidence-based medicine.
The principles of the use of fractal analysis in the problems of polarization mapping of microscopic images of biological preparations are considered. Myocardial tissue of the deceased with various pathological and necrotic changes was selected as the object of study. A model of the polycrystalline structure of such an object is proposed. Obtained maps of the ellipticity of the polarization of microscopic images of such an object. Within the framework of fractal analysis, the statistical moments of the 1st - 4th orders were found, which characterize the distribution of the logarithmic dependences of the power spectra of polarization ellipticity maps. The criteria for differentiation of various pathological states of myocardial tissue are determined.
This report contains theoretical algorithm for the differential representation of a phase-inhomogeneous biological object as a set of consecutively located optically anisotropic layers; theoretical algorithms for the decomposition of the Mueller-matrix of the diffuse biological layer in the basis of differential matrices of the 1st and 2nd orders; analytical relations for determining the magnitude of the set of elements of differential matrices of the 1st and 2nd orders;information and characteristics of optical schemes of experimental devices.
The results of the multifunctional Stokes polarimetric mapping of the manifestations of phase and amplitude anisotropy of histological sections of the internal organs of rats are presented. The methods of statistical analysis of vector-parametric images are used. Criteria for the differentiation of pathological conditions are obtained.
The paper consists of two parts. The first part presents short theoretical basics of the method of Jones-matrix mapping with the help of reference wave. It was provided experimentally measured coordinate distributions of modulus of Jones-matrix elements of polycrystalline film of bile. It was defined the values and ranges of changing of statistic moments, which characterize such distributions. The second part presents the data of statistic analysis of the distributions of matrix elements of polycrystalline film of urine of donors and patients with albuminuria. It was defined the objective criteria of differentiation of albuminuria.
The paper consists of two parts. The first part presents short theoretical basics of the method of azimuthally-invariant Mueller-matrix description of optical anisotropy of biological tissues. It was provided experimentally measured coordinate distributions of Mueller-matrix invariants (MMI) of linear and circular birefringences of skeletal muscle tissue. It was defined the values of statistic moments, which characterize the distributions of amplitudes of wavelet coefficients of MMI at different scales of scanning. The second part presents the data of statistic analysis of the distributions of amplitude of wavelet coefficients of the distributions of linear birefringence of myocardium tissue died after the infarction and ischemic heart disease. It was defined the objective criteria of differentiation of the cause of death.
The paper consists of two parts. The first part presents short theoretical basics of the method of azimuthally-invariant Mueller-matrix description of optical anisotropy of biological tissues. It was provided experimentally measured coordinate distributions of Mueller-matrix invariants (MMI) of linear and circular birefringences of skeletal muscle tissue. It was defined the values of statistic moments, which characterize the distributions of amplitudes of wavelet coefficients of MMI at different scales of scanning. The second part presents the data of statistic analysis of the distributions of amplitude of wavelet coefficients of the distributions of linear birefringence of myocardium tissue died after the infarction and ischemic heart disease. It was defined the objective criteria of differentiation of the cause of death.
In this paper, we present the results of a statistical analysis of polarization-interference images of optically thin histological sections of biological tissues and polycrystalline films of biological fluids of human organs. A new analytical parameter is introduced-the local contrast of the interference pattern in the plane of a polarizationinhomogeneous microscopic image of a biological preparation. The coordinate distributions of the given parameter and the sets of statistical moments of the first-fourth order that characterize these distributions are determined. On this basis, the differentiation of degenerative-dystrophic changes in the myocardium and the polycrystalline structure of the synovial fluid of the human knee with different pathologies is realized.
A new technique of Mueller-matrix mapping of polycrystalline structure of histological sections of biological tissues is suggested. The algorithms of reconstruction of distribution of parameters of linear and circular dichroism of histological sections liver tissue of mice with different degrees of severity of diabetes are found. The interconnections between such distributions and parameters of linear and circular dichroism of liver of mice tissue histological sections are defined. The comparative investigations of coordinate distributions of parameters of amplitude anisotropy formed by Liver tissue with varying severity of diabetes (10 days and 24 days) are performed. The values and ranges of change of the statistical (moments of the 1st – 4th order) parameters of coordinate distributions of the value of linear and circular dichroism are defined. The objective criteria of cause of the degree of severity of the diabetes differentiation are determined.
The paper presents the results of polarization manifestations of small - and Large-scale phase anisotropy of dead in consequence of ischemic heart disease (IHD) and acute coronary insufficiency (ACI) people myocardial tissue structures to differentiate information, the wavelet analysis method is used. The resulting maps of the of the polarizationcorrelation parameters distributions (the phase of the two-point first and second parameters of the Stokes vector) are analyzed in the framework of statistical approach. On this basis, the criteria for differential diagnosis of IHD and ACI cases have been determined.
In the approximation of weak phase fluctuations, a polarization-correlation method for diagnosing the optical anisotropy of small-scale optically anisotropic fibrillar structures of myocardial tissues is proposed.The algorithms of analytical description and experimental determination of the coordinate distributions of the value of the modulus and phase of "two-point" Stokes vector parameters are defined. Within the statistic, correlation and fractal analysis the possibility of differential diagnostics of slight changes in optical anisotropy of myocardial tissues of different pathological states. The comparative analysis of objective statistical, correlation and fractal analysis of distributions of "single-point" polarization azimuth and ellipticity and "two-point" Stokes vector parameters of polarization-inhomogeneous images of the studied histological sections demonstrated excellent accuracy ( Ac >90% ) of differential diagnostics of changes in the optical anisotropy myocardium tissues.
A model of generalized optical anisotropy of polycrystalline networks of albumin and globulin of human
brain liquor has been suggested. The polarization-phase method of spatial and frequency differentiation of
linear and circular birefringence coordinate distributions have been analytically substantiated. A set of criteria
of the dynamics of necrotic changes of polarization-phase images of liquor polycrystalline films for
determination of death coming prescription has been detected and substantiated.
The model of Mueller-matrix description of mechanisms of optical anisotropy that typical for polycrystalline layers of
the histological sections of biological tissues and fluids - optical activity, birefringence, as well as linear and circular
dichroism - is suggested. Within the statistical analysis distributions quantities of linear and circular birefringence and
dichroism the objective criteria of differentiation of myocardium histological sections (determining the cause of death);
films of blood plasma (liver pathology); peritoneal fluid (endometriosis of tissues of women reproductive sphere); urine
(kidney disease) were determined. From the point of view of probative medicine the operational characteristics
(sensitivity, specificity and accuracy) of the method of Mueller-matrix reconstruction of optical anisotropy parameters
were found.
A new azimuthally stable polarimetric method for processing of microscopic images of optically anisotropic structures of
different biological layers histological sections is proposed. A new model of phase anisotropy definition of biological
tissues by using superposition of Mueller matrices of linear birefringence and optical activity is proposed. The matrix
element M44 has been chosen as the main information parameter, which value is independent of rotation angle of both
sample and probing beam polarization plane.
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