The new aqueous gel compositions based on 5-aminolevulinic acid (ALA) for fluorescent diagnostics and
photodynamic therapy of superficial diseases have been elaborated. Biodegradable polymer was used as gel-forming
component and some additives - solubilizer, emulgator etc - were used to improve distribution uniformity and
penetration ability. These new compositions represent colorless and transparent gels which are long-term stable at the
storage temperature of ≤5°C.
The effectiveness of aqueous gels as ALA delivery systems is demonstrated. After topical administration of the gel
at the skin with Ehrlich tumor inoculated subcutaneously, ALA effectively induces the synthesis of protoporphyrine IX
(PPIX) in the skin and in the tumor. Intensity of ALA-induced PPIX fluorescence grows with increasing ALA
concentration in the gel and time of gel application. The deepness of ALA penetration in the tumor reaches 5-6 mm after
4 h gel application.
A distribution of ALA-induced PPIX in mouse tumor tissue after 4 h gel application depends on ALA concentration
in gel. Intensity of PPIX fluorescence in deep-located part of tumor was substantially higher with gel containing 20%
ALA than with ones containing 10% or 5% ALA.
Choroidal metastasis (CM) are more and more spreading type of eye's neoplasma. The frequency of CM is
increasing with prolonging of cancer patients' life. And it makes worse the quality of their life because blindness.
Photodynamic therapy (PDT) is very delicate modality, which can be used for this purpose. The aim of this work was to
open the possibility and to determine the efficacy of photodynamic therapy (PDT) in the treatment of patients with CM.
PDT was performed simultaneously with standard chemotherapy in 8 oncological patients with CM. We used
photosensitizer Photosens in doses of 0.3 mg/kg and light doses 150 J/cm2 (675 nm). PDT was performed in the some
stances. Its are ranged from 7 to 10. Complete tumor regression was achieved in 6 cases. The high retina ablation was
developed in one case. And in one case effect was not complete: tumor size reduced from 5 mm to 3 mm of thickness.
We didn't notice any recurrence for 6-18 months follow-up. PDT is modality that could to be used in the in the
combined treatment of the CM.
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