Mr. Neal Saini Profile

Neal Saini

at Medical Univ of South Carolina

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 22 February 2019 Paper

Nondestructive method for chondrocyte viability assessment in articular cartilage tissues with nonlinear optical microscopy

Yang Li, Beckham Watkins, Elizabeth Nadeau, Russell Reeves, Razeen Basunia, Neal Saini, Xun Chen, Steven Gannon, Vincent Pellegrini, Hai Yao, Tong Ye

Proceedings Volume 10882, 108822L (2019) https://doi.org/10.1117/12.2510746

KEYWORDS: Auto-fluorescence imaging, Cartilage, Tissues, Microscopy, Optical microscopy, Second-harmonic generation, Nondestructive evaluation, Collagen

Read Abstract +

Covering the ends of long bones, articular cartilage provides a smooth, lubricated surface to absorb impact and distribute loads during movement so that underlying bone is protected. This function is facilitated by a complex and well-organized extracellular matrix (ECM). Being the only cell type in articular cartilage, chondrocytes are responsible for maintaining the homeostasis of the cartilage ECM; as such, the viability of chondrocytes is a critical parameter to reflect the quality of the cartilage. Most prevalent cell viability assays rely on dye staining and thereby cannot be performed for longitudinal monitoring or in-vivo assessment. Here we demonstrate that two-photon autofluorescence (TPAF) microscopy distinguishes live cells from dead cells in intact ex-vivo cartilage tissues, which provides a non-invasive method to assess cell viability. In our study, the endogenous fluorophores such as nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD) were used to image chondrocytes in cartilages on rat tibia condyles immediately after harvesting. Second harmonic generation (SHG) imaging was also performed to examine the integrity of the extracellular and pericellular matrix. On the same tissue, the cell viability assay with Calcein-AM and Ethidium homodimer-1 (EthD-1) labeling was used as a gold standard to identify live or dead cells. We found that live cells presented stronger NAD(P)H fluorescence than dead cells in general and were readily identified if pseudo colors were used for showing two-channel images. Owing to its non-destructive nature, this method holds the potential value in assessing cell viability of engineered or living tissues without dye labeling.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years