Ms. Nicole Prent Profile

Nicole Prent

at Univ of Toronto Mississauga

SPIE Involvement:

Author

Publications (9)

SPIE Journal Paper | 7 May 2014 Open Access

Second harmonic generation polarization properties of myofilaments

Masood Samim, Nicole Prent, Daniel Dicenzo, Bryan Stewart, Virginijus Barzda

JBO, Vol. 19, Issue 05, 056005, (May 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.5.056005

KEYWORDS: Second-harmonic generation, Polarization, Harmonic generation, Head, Microscopy, Molecules, Microscopes, Birefringence, Proteins, Polarizers

Read Abstract +

DOWNLOAD PAPER

SPIE Journal Paper | 1 March 2010 Open Access

Nonlinear multicontrast microscopy of hematoxylin-and-eosin-stained histological sections

Adam Tuer, Danielle Tokarz, Nicole Prent, Richard Cisek, Jennifer Alami, Daniel Dumont, Ludmilla Bakueva, John Rowlands, Virginijus Barzda

JBO, Vol. 15, Issue 02, 026018, (March 2010) https://doi.org/10.1117/12.10.1117/1.3382908

KEYWORDS: Tissues, Luminescence, Absorption, Microscopy, Nonlinear optics, Microscopes, Tissue optics, Second-harmonic generation, Picosecond phenomena, Aluminum

Read Abstract +

DOWNLOAD PAPER

SPIE Journal Paper | 1 July 2008 Open Access

Intermyofilament dynamics of myocytes revealed by second harmonic generation microscopy

Nicole Prent, Chantal Green, Catherine Greenhalgh, Richard Cisek, Arkady Major, Bryan Stewart, Virginijus Barzda

JBO, Vol. 13, Issue 04, 041318, (July 2008) https://doi.org/10.1117/12.10.1117/1.2950316

KEYWORDS: Second-harmonic generation, Molecules, Microscopy, Harmonic generation, Microscopes, Roads, Constructive interference, Diagnostics, Physical sciences, Physics

Read Abstract +

DOWNLOAD PAPER

Proceedings Article | 7 February 2008 Paper

Ultrafast Yb:KGd(WO4)2 laser for multimodal biomedical imaging with reduced photodamage

Arkady Major, Richard Cisek, Adam Tuer, Nicole Prent, Virginijus Barzda

Proceedings Volume 6881, 688108 (2008) https://doi.org/10.1117/12.763953

KEYWORDS: Luminescence, Mirrors, Biomedical optics, Femtosecond phenomena, Signal detection, Crystals, Laser development, Absorption, Harmonic generation, Imaging systems

Read Abstract +

Proceedings Article | 8 September 2006 Paper

Dynamic investigation of Drosophila myocytes with second harmonic generation microscopy

Catherine Greenhalgh, Bryan Stewart, Richard Cisek, Nicole Prent, Arkady Major, Virginijus Barzda

Proceedings Volume 6343, 634308 (2006) https://doi.org/10.1117/12.706559

KEYWORDS: Second-harmonic generation, Harmonic generation, Microscopy, Microscopes, Luminescence, Crystals, Femtosecond phenomena, Oscillators, Multiphoton microscopy, Potassium

Read Abstract +

Proceedings Article | 8 September 2006 Paper

A diode-pumped high power extended cavity femtosecond Yb:KGW laser: from development to applications in nonlinear microscopy

Arkady Major, Richard Cisek, Catherine Greenhalgh, Nicole Prent, Bryan Stewart, Virginijus Barzda

Proceedings Volume 6343, 634345 (2006) https://doi.org/10.1117/12.707936

KEYWORDS: Laser development, Crystals, Femtosecond phenomena, Mirrors, Laser crystals, Pulsed laser operation, Semiconductor lasers, Microscopy, Mode locking, Signal detection

Read Abstract +

Proceedings Article | 8 September 2006 Paper

Optimization of nonlinear excitation for reducing light-induced changes in photosynthetic systems during imaging with multimodal microscopy

Richard Cisek, Arkady Major, Nicole Prent, Catherine Greenhalgh, Virginijus Barzda

Proceedings Volume 6343, 634307 (2006) https://doi.org/10.1117/12.706555

KEYWORDS: Second-harmonic generation, Luminescence, Quenching (fluorescence), Microscopes, Nonlinear optics, Microscopy, Harmonic generation, Sapphire lasers, Imaging systems, Complex systems

Read Abstract +

Nonlinear microscopy is a very attractive tool for studying photosynthetic organisms on cellular and subcellular levels. The multimodal microscope can be employed to image photosynthetic structures simultaneously with multiphoton excitation fluorescence (MPF), second harmonic generation (SHG), and third harmonic generation (THG) contrast mechanisms. Although the multimodal nonlinear microscope delivers invaluable information about the structure, spectroscopic properties, and functional dynamics of photosynthetic systems, the prompt light-induced changes of highly light sensitive pigment-protein complexes complicate the extensive study of photosynthetic organisms. In this work, we investigated the extent of light-induced changes in chloroplasts from higher plants by imaging with a Ti:Sapphire femtosecond laser and a Yb-ion doped potassium gadolinium tungstate (Yb:KGW) femtosecond laser. The Ti:Sapphire laser delivered 800 nm wavelength and ~25 fs duration pulses at a 26.7 MHz repetition rate. In comparison, the Yb:KGW laser provided a 1042 nm wavelength, ~200 fs pulses at a repetition rate of 14.6 MHz. The 800 nm pulses predominantly excited chlorophyll pigments via two-photon excitation, while 1042 nm excitation resulted in two-photon absorption of carotenoids. The induced fluorescence quenching, and decrease in SHG and THG signal was much stronger when imaged with a Ti:Sapphire laser. Prolonged imaging of up to tenths of minutes with the Yb:KGW laser did not result in appreciable changes of all three nonlinear signals. The difference in the light-induced changes most probably appears due to the difference in excited state dynamics following chlorophyll or carotenoid excitation. The slow component of MPF and THG changes as well as change in SHG reflects the light-induced macroorganization of the grana, while the fast MPF and THG component is tentatively attributed to the generation of quenchers from chlorophyll molecules. The success in imaging photosynthetic samples for prolonged periods of time with a Yb:KGW laser opens a new window of opportunity for thorough in vivo investigations of photosynthetic structures.

Proceedings Article | 14 October 2005 Paper

Time and structural crosscorrelation image analysis of microscopic volumes, simultaneously recorded with second harmonic generation, third harmonic generation, and multiphoton excitation fluorescence microscopy

Catherine Greenhalgh, Richard Cisek, Nicole Prent, Arkady Major, Juerg Aus de Au, Jeff Squier, Virginijus Barzda

Proceedings Volume 5969, 59692F (2005) https://doi.org/10.1117/12.628786

KEYWORDS: Image analysis, Luminescence, Harmonic generation, Microscopy, Image visualization, Image segmentation, Multiphoton microscopy, Microscopes, Visualization, 3D image processing

Read Abstract +

Proceedings Article | 1 October 2005 Paper

Applications of nonlinear microscopy for studying the structure and dynamics in biological systems

Nicole Prent, Richard Cisek, Catherine Greenhalgh, Raymond Sparrow, Neeresh Rohitlall, Maike-Svenja Milkereit, Chantal Green, Virginijus Barzda

Proceedings Volume 5971, 597106 (2005) https://doi.org/10.1117/12.628801

KEYWORDS: Second-harmonic generation, Microscopy, Harmonic generation, Nonlinear optics, Luminescence, Interfaces, Signal generators, Multiphoton microscopy, Microscopes, Signal detection

Read Abstract +

Showing 5 of 9 publications

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Keywords/Phrases

Search In:

Publication Years