Ms. Paula Villarreal Profile

Paula Villarreal

SPIE Involvement:

Author

Publications (7)

Proceedings Article | 13 March 2024 Presentation

Widefield multispectral imaging to evaluate spectral biomarkers associated with oral epithelial dysplasia and squamous cell carcinoma in a hamster buccal model and human clinical samples

Paula Villarreal, Suimin Qiu, Orly Coblens, Tyler Peterson, Bofan Song, Xingde Li, Rongguang Liang, Gracie Vargas

Proceedings Volume PC12818, (2024) https://doi.org/10.1117/12.3003202

KEYWORDS: Multispectral imaging, Tumor growth modeling, Statistical modeling, Cancer detection, Tissues, Autofluorescence, Tunable filters, Neck, Liquid crystals, Light emitting diodes

Read Abstract +

Proceedings Article | 13 March 2024 Presentation

Benchtop to handheld: characterization and optimization of a ring illuminator/detector in a handheld endogenous fluorescence imaging probe

Paula Villarreal, Tyler Peterson, Bofan Song, Rongguang Liang, Gracie Vargas

Proceedings Volume PC12833, PC1283304 (2024) https://doi.org/10.1117/12.3009704

KEYWORDS: Fluorescence, Light sources and illumination, Imaging systems, Autofluorescence imaging, Signal detection, Light emitting diodes, LED lighting, Fluorescence imaging, Diseases and disorders, Ultraviolet light emitting diodes

Read Abstract +

Proceedings Article | 6 March 2023 Presentation

Design and characterization of handheld multiphoton probe for intraoral imaging

Tyler Peterson, Hongzhang Ma, Paula Villarreal, Xingde Li, Gracie Vargas, Rongguang Liang

Proceedings Volume PC12370, PC1237007 (2023) https://doi.org/10.1117/12.2657635

KEYWORDS: Image resolution, Image quality, Tissues, Prisms, Natural surfaces, Multiphoton fluorescence microscopy, Manufacturing, Design for manufacturability, Cancer, Cameras

Read Abstract +

Proceedings Article | 6 March 2023 Presentation

Analysis considerations for multimodal based approaches in diagnostics

Paula Villarreal, Suimin Qiu, Xingde Li, Rongguang Liang, Gracie Vargas

Proceedings Volume PC12370, PC1237008 (2023) https://doi.org/10.1117/12.2657771

KEYWORDS: Diagnostics, Optical imaging, Multiphoton microscopy, Multimodal imaging, Luminescence, Tumor growth modeling, Tissues, Optical sensing, Cancer

Read Abstract +

Proceedings Article | 5 March 2021 Presentation

Designing a multimodal imaging workflow for large area fluorescence with nonlinear optical microscopy for oral neoplasia detection: preclinical model and clinical sample study

Rahul Pal, Paula Villarreal, Xiaoying Yu, Suimin Qiu, Orly Coblens, Gracie Vargas

Proceedings Volume 11622, 116220I (2021) https://doi.org/10.1117/12.2591286

Read Abstract +

Proceedings Article | 5 March 2021 Presentation

Use of preclinical models alongside clinical tissues to develop a noninvasive multimodal fluorescence detection approach for oral neoplasia detection

Paula Villarreal, Rahul Pal, Xiaoying Yu, Orly Coblens, Suimin Qiu, Rongguang Liang, Xingde Li, Gracie Vargas

Proceedings Volume 11633, 116330I (2021) https://doi.org/10.1117/12.2591126

Read Abstract +

SPIE Journal Paper | 16 November 2020 Open Access

Multimodal widefield fluorescence imaging with nonlinear optical microscopy workflow for noninvasive oral epithelial neoplasia detection: a preclinical study

Rahul Pal, Paula Villarreal, Xiaoying Yu, Suimin Qiu, Gracie Vargas

JBO, Vol. 25, Issue 11, 116008, (November 2020) https://doi.org/10.1117/12.10.1117/1.JBO.25.11.116008

KEYWORDS: Inflammation, Roentgenium, Luminescence, In vivo imaging, Multimodal imaging, Microscopy, Tissues, Optical microscopy, Optical imaging, Tumors

Read Abstract +

Significance: Early detection of epithelial cancers and precancers/neoplasia in the presence of benign lesions is challenging due to the lack of robust in vivo imaging and biopsy guidance techniques. Label-free nonlinear optical microscopy (NLOM) has shown promise for optical biopsy through the detection of cellular and extracellular signatures of neoplasia. Although in vivo microscopy techniques continue to be developed, the surface area imaged in microscopy is limited by the field of view. FDA-approved widefield fluorescence (WF) imaging systems that capture autofluorescence signatures of neoplasia provide molecular information at large fields of view, which may complement the cytologic and architectural information provided by NLOM. Aim: A multimodal imaging approach with high-sensitivity WF and high-resolution NLOM was investigated to identify and distinguish image-based features of neoplasia from normal and benign lesions. Approach:In vivo label-free WF imaging and NLOM was performed in preclinical hamster models of oral neoplasia and inflammation. Analyses of WF imaging, NLOM imaging, and dual modality (WF combined with NLOM) were performed. Results: WF imaging showed increased red-to-green autofluorescence ratio in neoplasia compared to inflammation and normal oral mucosa (p < 0.01). In vivo assessment of the mucosal tissue with NLOM revealed subsurface cytologic (nuclear pleomorphism) and architectural (remodeling of extracellular matrix) atypia in histologically confirmed neoplastic tissue, which were not observed in inflammation or normal mucosa. Univariate and multivariate statistical analysis of macroscopic and microscopic image-based features indicated improved performance (94% sensitivity and 97% specificity) of a multiscale approach over WF alone, even in the presence of benign lesions (inflammation), a common confounding factor in diagnostics. Conclusions: A multimodal imaging approach integrating strengths from WF and NLOM may be beneficial in identifying oral neoplasia. Our study could guide future studies on human oral neoplasia to further evaluate merits and limitations of multimodal workflows and inform the development of multiscale clinical imaging systems.

DOWNLOAD PAPER

Showing 5 of 7 publications

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Keywords/Phrases

Search In:

Publication Years