An imaging device based on fluorescence for in vivo detection of oral cancer has been developed. Images collected from cancerous patients and normal volunteers have shown a clear difference in fluorescence intensity
We report here a comparative study for oral precancer detection using fluorescence spectroscopy on human oral cavity and body fluid saliva. In-vivo detection of oral precancer has been carried out by an in-house developed handheld system and detection on human saliva has been performed by in-house developed compact set-up. The Study has been conducted on three groups of patients: oral squamous cell carcinoma (OSCC), dysplastic (precancer), and control (normal). Fluorescence spectra recorded from oral buccal mucosa (BM) consist of major and minor bands of flavin adenine dinucleotide (FAD) and porphyrins near 500, 634, 689 and 703 nm. Spectra recorded from human saliva also showed the same bands in addition to a new band near 437 nm. Receiver operating characteristic (ROC) analysis has been used to evaluate diagnostic performance. Ratios of the peak values of intensities of porphyrin (634 nm) to FAD (500 nm) bands from the spectra of BM and area under the entire spectra of saliva samples are taken as discriminating parameters to differentiate the groups. Obtained results with human saliva are found to be similar as from BM and we conclude that it may be used as a substitute diagnostic medium of early oral cancer detection.
A comparative study has been accomplished between diagnostic mediums: human oral tissue and saliva for oral cancer detection on three groups: oral squamous cell carcinoma (OSCC), dysplastic, and control (normal) by using Stokes shift (SS) spectroscopy (SSS) at ▵λ of 120 nm, which is the Stokes shift of nicotinamide adenine dinucleotide (NADH). SS spectra obtained from tissue and saliva consist of major bands of collagen, tryptophan, NADH and minor bands of flavin adenine dinucleotide (FAD) and porphyrin. Principal component analysis (PCA) has been performed on the data sets of SS spectra for discrimination by dimension reduction. Linear discriminant analysis (LDA) has been applied on the PC scores to compute linear discriminant (LD) scores. Kernel probability density functions of LD scores are plotted to show how LD scores of each group are separated from one another. LD scores for oral tissue differentiates OSCC to normal, dysplasia to normal, and OSCC to dysplasia with sensitivities 100 %, 85 %, 94 % and specificities 88 %, 88 %, 89 % with the accuracy of 95 %, 87 % and 92 %. On the other hand for saliva, it differentiates respective groups with sensitivities 91 %, 82 %, 91 % and specificities 100 %, 88 %, 82 % with the accuracy of 95 %, 85 % and 87 %. Obtained results with human saliva are as prominent as oral tissue and we conclude that it may be used as a substitute diagnostic medium. In addition SS spectroscopy instead of fluorescence spectroscopy at 120 nm shift appears to be an important tool for in vivo detection of early oral cancer.
Throat precancer detection using fluorescence from human saliva is reported here. It may be noted that accessing the throat for investigation is cumbersome and use of saliva as a diagnostic medium may ease the process. The study has been conducted on three groups of patients: oral squamous cell carcinoma (OSCC), dysplasia, and normal (control). An in-house developed compact set-up has been used for fluorescence measurements. The compact system consist of a 375 nm laser diode, collimating lens, long pass filter, fibers, and cuvette holder. Major and minor bands of flavin adenine dinucleotide (FAD) and porphyrin are observed in the spectra. A receiver operating characteristic (ROC) analysis has been used to evaluate the diagnostic performance. Area under the spectra has been chosen for discrimination among the groups and is able to differentiate OSCC to normal, dysplasia to normal, and OSCC to dysplasia with sensitivities 100% (48/48), 92% (32/35), 77% (37/48), and specificities 96% (50/52), 96% (50/52), 89% (31/35) with the accuracy of 98%, 94% and 82% respectively. Sensitivity and specificity, when differentiating OSCC to normal and dysplasia to normal, are significantly large, which indicates that human saliva may be an excellent diagnostic medium for early detection of throat cancer.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.