Mr. Quinn M. Barber Profile

Quinn M. Barber

at Univ of Alberta

SPIE Involvement:

Author

Publications (5)

Proceedings Article | 27 April 2016 Presentation

Engineering a near-infrared dark chromoprotein optimized for photoacoustic imaging (Conference Presentation)

Yan Li, Quinn Barber, Robert Paproski, Jhon Enterina, Erik Rodriguez, Roger Tsien, Robert Campbell, Roger Zemp

Proceedings Volume 9708, 97081Z (2016) https://doi.org/10.1117/12.2209150

KEYWORDS: Photoacoustic imaging, Photoacoustic spectroscopy, Absorption, Imaging systems, Visual process modeling, Luminescence, Quantum efficiency, Near infrared, Fluorescent proteins, Proteins

Read Abstract +

Proceedings Article | 15 March 2016 Paper

Multiple-illumination photoacoustic tomography

Quinn Barber, Roger Zemp

Proceedings Volume 9708, 97084G (2016) https://doi.org/10.1117/12.2209387

KEYWORDS: Ultrasonography, Tomography, Scattering, Photoacoustic tomography, Anisotropy, Photoacoustic spectroscopy, Algorithm development, Photoacoustic imaging, Data modeling, Breast imaging, Transducers, Ultrasound tomography, Absorption, Composites

Read Abstract +

SPIE Journal Paper | 21 October 2015 Open Access

Validating tyrosinase homologue melA as a photoacoustic reporter gene for imaging Escherichia coli

Robert Paproski, Yan Li, Quinn Barber, John Lewis, Robert Campbell, Roger Zemp

JBO, Vol. 20, Issue 10, 106008, (October 2015) https://doi.org/10.1117/12.10.1117/1.JBO.20.10.106008

KEYWORDS: Bacteria, Photoacoustic spectroscopy, Tissue optics, Photoacoustic imaging, Scattering, Bioluminescence, Signal detection, Luminescence, Imaging systems, Visualization

Read Abstract +

To understand the pathogenic processes for infectious bacteria, appropriate research tools are required for replicating and characterizing infections. Fluorescence and bioluminescence imaging have primarily been used to image infections in animal models, but optical scattering in tissue significantly limits imaging depth and resolution. Photoacoustic imaging, which has improved depth-to-resolution ratio compared to conventional optical imaging, could be useful for visualizing melA-expressing bacteria since melA is a bacterial tyrosinase homologue which produces melanin. Escherichia coli-expressing melA was visibly dark in liquid culture. When melA-expressing bacteria in tubes were imaged with a VisualSonics Vevo LAZR system, the signal-to-noise ratio of a 9× dilution sample was 55, suggesting that ∼20 bacteria cells could be detected with our system. Multispectral (680, 700, 750, 800, 850, and 900 nm) analysis of the photoacoustic signal allowed unmixing of melA-expressing bacteria from blood. To compare photoacoustic reporter gene melA (using Vevo system) with luminescent and fluorescent reporter gene Nano-lantern (using Bruker Xtreme In-Vivo system), tubes of bacteria expressing melA or Nano-lantern were submerged 10 mm in 1% Intralipid, spaced between <1 and 20 mm apart from each other, and imaged with the appropriate imaging modality. Photoacoustic imaging could resolve the two tubes of melA-expressing bacteria even when the tubes were less than 1 mm from each other, while bioluminescence and fluorescence imaging could not resolve the two tubes of Nano-lantern-expressing bacteria even when the tubes were spaced 10 mm from each other. After injecting 100-μL of melA-expressing bacteria in the back flank of a chicken embryo, photoacoustic imaging allowed visualization of melA-expressing bacteria up to 10-mm deep into the embryo. Photoacoustic signal from melA could also be separated from deoxy- and oxy-hemoglobin signal observed within the embryo and chorioallantoic membrane. Our results suggest that melA is a useful photoacoustic reporter gene for visualizing bacteria, and further work incorporating photoacoustic reporters into infectious bacterial strains is warranted.

DOWNLOAD PAPER

Proceedings Article | 11 March 2015 Paper

Blanket illumination vs scanned-mosaicking imaging schemes for wide-area photoacoustic tomography

Quinn Barber, Tyler Harrison, Roger Zemp

Proceedings Volume 9323, 93232V (2015) https://doi.org/10.1117/12.2079950

KEYWORDS: Signal to noise ratio, Monte Carlo methods, Transducers, Tissues, Photoacoustic tomography, Ultrasonics, Scattering, Acquisition tracking and pointing, Photoacoustic spectroscopy, Laser scattering

Read Abstract +

Proceedings Article | 11 March 2015 Paper

Validating tyrosinase homologue MelA as a photoacoustic reporter gene for imaging escherichia coli

Robert Paproski, Yan Li, Quinn Barber, John Lewis, Robert Campbell, Roger Zemp

Proceedings Volume 9323, 93232H (2015) https://doi.org/10.1117/12.2079638

KEYWORDS: Bacteria, Photoacoustic spectroscopy, Tissues, Visualization, Blood, Tissue optics, Imaging systems, Photoacoustic imaging, Proteins, Signal detection

Read Abstract +

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Keywords/Phrases

Search In:

Publication Years