Collection of biopsies from the most pathologically advanced region is critical for histopathological assessment of potentially cancerous sites in the lung. However, current applications are limited in their ability to simultaneously image and collect samples in subsegmental airways. We demonstrate a suction-snare device guided with optical coherence tomography and autofluorescence imaging (OCT-AFI) to improve diagnostic yield in these airways. Biopsies collected in healthy ex-vivo porcine airways are shown to retain structural and functional information. Feasibility is demonstrated in an ex-vivo porcine model to assess tissue abnormality prior to biopsy collection.
We have previously demonstrated multimodal optical coherence tomography and autofluorescence imaging (OCT-AFI) in the distal airways of the lung. To combine the two modalities into a single-fiber endoscope, we use double-clad fibers, which causes additional blurred OCT images from the fibers' higher-order modes. Recently, we established multipath contrast imaging (MCI) which leverages these higher-order images to elucidate angular backscattering of tissue. MCI can be generated retroactively; we seek to re-evaluate images from our in vivo OCT-AFI lung cancer study. Early MCI results demonstrate high contrast in healthy tissue compared to blood, and for a histologically confirmed adenocarcinoma.
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