Lensfree digital holographic technique can become a powerful microscopic solution by adequately adapting a super resolution(SR) method together with an advanced phase retrieval algorithm. However, it comes at the cost of acquiring multiple images as well as processing large volume of data. Here, we present a multi-height based SR technique that can maximize the signal to noise ratio and the resolution, approximately 2.5 times over the actual pixel size of an image sensor, while minimizing computational cost by utilizing the much less set of the sub-pixel shifted images compared to the conventional SR methods.
We demonstrate a high resolution lens-free holographic microscopy in reflection geometry based on a pixel super resolution (SR) method. The lens-free microscopy uses a novel Michelson geometry suitable to image reflective samples with the large field of view, while the Fourier domain SR technique is applied to obtain the high resolution hologram, achieving the sub-pixel resolution of 1.2 μm in the USAF reflection target by utilizing the randomly shifted low resolution images. The proposed compact microscopy technique enables to provide high resolution amplitude and phase imaging, those are suitable for biology and semiconductor imaging applications.
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